Phylogenetical metabolite logical algorithm: A test using phylogenetic metabolite analysis using TLC in some convolvulaceae members.
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Date
2010-11
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Abstract
Chemical substances act as vital substances occurring in living organisms
and can be studied through various biotechnological approaches. The characteristic
metabolites of a plant species may reflect evolution towards the resistance produced to
environment. Evolvulus alsinoides, Evolvulus nummularius and Merrimia tridentata
belongs to convolvulaceae members has been taken as experimental plants in the present
study. Thin layer chromatography (TLC) has been used as a data collection technique for
identification of similar organisms by logical method, from various Rf values occurred by
experimentation. Based on phylogenetic analysis using UPGMA method, E.nummularius
has shown good matches with both E.alsinoides and M.tridentata. Hence E.nummularius
may be showing evolutionary link between E.alsinoides and M.tridentata. Seventeen
compounds are visualized with similar Rf values between all these plant members. Hence
most of the data are observed to be having similar compounds, which makes to be placed
in same family (convolvulaceae).
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Keywords
Convolvulaceae, Thin layer chromatography, logical method, MEGA software, Phylogeny
Citation
Kaladhar DSVGK, Harasreeramulu S. Phylogenetical metabolite logical algorithm: A test using phylogenetic metabolite analysis using TLC in some convolvulaceae members. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Nov-Dec; 1(3): 926-934.