Evaluation of the Anti-inflammatory Effects of Methanol Extract of Rauwofia vomitera Leaves (MERVL) Using In-vivo Based Assays
dc.contributor.author | Celestine, Ugwu Obiora | en_US |
dc.contributor.author | Jacinta, Ukpabi-Ugo Chigozie | en_US |
dc.contributor.author | Lilian, Asadu Chidinma | en_US |
dc.contributor.author | Jude, Ali Ibeabuchi | en_US |
dc.contributor.author | Keneolisa, Asogwa Felix | en_US |
dc.contributor.author | Walter, Ugodi Gerald | en_US |
dc.contributor.author | Emmanuel, Obasi Uchenna | en_US |
dc.contributor.author | Gloria, Ogbonna Chidera | en_US |
dc.contributor.author | Kingsley, Nwokedi Chukwuebuka | en_US |
dc.contributor.author | Assumpta, Anosike Chioma | en_US |
dc.date.accessioned | 2024-09-24T06:22:55Z | |
dc.date.available | 2024-09-24T06:22:55Z | |
dc.date.issued | 2024-05 | |
dc.description.abstract | Background of the Study: The response of the body to harmful stimuli, such as tissue damage or a pathogenic infection is known as inflammation. Rauwofia vomitera is a medicinal plant that is native to large areas of tropical Africa and it belongs to the family of Apocynaceae. It is a medicinal herb used in traditional Nigerian medicine to treat a variety of diseases, including inflammatory disorders.Aim of the Study:The present study investigated the anti-inflammatory effects of methanol extract of Rauwofia vomitera leaves (MERVL) using in-vivo based assays. Methodology: The anti-inflammatory effect of (MERVL) was ascertained using the Egg albumin -induced inflammation using in vivo assay. A total of thirty (30) adult male albino rats (130-170g) were divided into five (5) groups of six (6) rats each for the in-vivo anti-inflammatory assay. Group 1 normal; Group 2 was given 10 mg/kg body weight of Indomethacin and Groups 3, 4 and 5 were given 100, 200 and 400 mg/kg body weight of MERVL) respectively. Acute toxicity was carried out using standard method.Place and Duration of the Study: Department of Pharmacology Lab, Enugu State of University of Science and Technology Agbani Nigeria, between March 2021 and August 2021.Results: The percentage yield of the extract was 10.8%. The result of the quantitative and qualitative phytochemical screening of methanol extract of R. vomitoria leaves showed that Phenol (1590 � 55.66) Flavonoid (1168 � 11.43) and Tannin (1466 � 40.73) were present in high concentration whereas, Alkaloid (549 � 4.21) Glycoside (315 � 61.38) and Terpenoid (162 � 1.38) were present in moderate concentration. Saponin (1.16 � 0.23) and Steroid (0.84 � 0.03) were present in low concentrations. Acute toxicity tests showed no toxicity and mortality at doses up to 5000 mgkg-1. A significant (P < 0.05) reduction in the mean paw oedema was observed for all the treatment groups from 1 hour to 12 hours when compared to the normal group. The result shown that groups treated with 100, 200 and 400 mg/kg b.w of extract inhibit the inflammation in a non-dose dependent manner with percentage mobilization of 5.7%, 14.1% and 6.2% respectively.Conclusion: The study's results demonstrate that MERVL exhibits remarkable anti-inflammatory and anti-oxidant activities and the extract yielded a better reduction in edema and increases mobilization in leukocyte in response to agar suspension. It also demonstrates that MERVL has a modulatory effect on the vascular changes that accompany inflammation. The plant's anti-inflammatory properties could potentially result from the interaction of different photochemical substances present. The findings suggest that, if used, the plant may serve as a source of anti-inflammatory agents. | en_US |
dc.identifier.affiliations | Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria and Department of Biochemistry, Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria | en_US |
dc.identifier.affiliations | Department of Biochemistry, Faculty of Natural Sciences, Micheal Okpara University of Agriculture Umudike Abia State, Nigeria | en_US |
dc.identifier.affiliations | Department of Medical Biochemistry, College of Medicine, Esut, Parklane, Nigeria | en_US |
dc.identifier.affiliations | Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria | en_US |
dc.identifier.affiliations | Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria | en_US |
dc.identifier.affiliations | Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria | en_US |
dc.identifier.affiliations | Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria | en_US |
dc.identifier.affiliations | Department of Biochemistry, Faculty of Natural and Applied Sciences, State University of Medical and Applied Sciences, Igbo Eno. Enugu State, Nigeria | en_US |
dc.identifier.affiliations | Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria | en_US |
dc.identifier.affiliations | Department of Biochemistry, Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria | en_US |
dc.identifier.citation | Celestine Ugwu Obiora, Jacinta Ukpabi-Ugo Chigozie, Lilian Asadu Chidinma, Jude Ali Ibeabuchi, Keneolisa Asogwa Felix, Walter Ugodi Gerald, Emmanuel Obasi Uchenna, Gloria Ogbonna Chidera, Kingsley Nwokedi Chukwuebuka, Assumpta Anosike Chioma. Evaluation of the Anti-inflammatory Effects of Methanol Extract of Rauwofia vomitera Leaves (MERVL) Using In-vivo Based Assays . International Journal of Medical Research & Health Sciences. 2024-May; 33(5): 70-78 | en_US |
dc.identifier.issn | 2231-086X | |
dc.identifier.place | India | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/226840 | |
dc.language | en | en_US |
dc.publisher | Ms. M. B. Mondal | en_US |
dc.relation.issuenumber | 5 | en_US |
dc.relation.volume | 33 | en_US |
dc.source.uri | https://doi.org/10.9734/ijbcrr/2024/v33i5877 | en_US |
dc.subject | Rauwofia VOMITERA | en_US |
dc.subject | egg albumin | en_US |
dc.subject | anti-inflammatory | en_US |
dc.subject | leucocyte mobilization | en_US |
dc.subject | methanol extract | en_US |
dc.title | Evaluation of the Anti-inflammatory Effects of Methanol Extract of Rauwofia vomitera Leaves (MERVL) Using In-vivo Based Assays | en_US |
dc.type | Journal Article | en_US |
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