Evaluation of the Anti-inflammatory Effects of Methanol Extract of Rauwofia vomitera Leaves (MERVL) Using In-vivo Based Assays

dc.contributor.authorCelestine, Ugwu Obioraen_US
dc.contributor.authorJacinta, Ukpabi-Ugo Chigozieen_US
dc.contributor.authorLilian, Asadu Chidinmaen_US
dc.contributor.authorJude, Ali Ibeabuchien_US
dc.contributor.authorKeneolisa, Asogwa Felixen_US
dc.contributor.authorWalter, Ugodi Geralden_US
dc.contributor.authorEmmanuel, Obasi Uchennaen_US
dc.contributor.authorGloria, Ogbonna Chideraen_US
dc.contributor.authorKingsley, Nwokedi Chukwuebukaen_US
dc.contributor.authorAssumpta, Anosike Chiomaen_US
dc.date.accessioned2024-09-24T06:22:55Z
dc.date.available2024-09-24T06:22:55Z
dc.date.issued2024-05
dc.description.abstractBackground of the Study: The response of the body to harmful stimuli, such as tissue damage or a pathogenic infection is known as inflammation. Rauwofia vomitera is a medicinal plant that is native to large areas of tropical Africa and it belongs to the family of Apocynaceae. It is a medicinal herb used in traditional Nigerian medicine to treat a variety of diseases, including inflammatory disorders.Aim of the Study:The present study investigated the anti-inflammatory effects of methanol extract of Rauwofia vomitera leaves (MERVL) using in-vivo based assays. Methodology: The anti-inflammatory effect of (MERVL) was ascertained using the Egg albumin -induced inflammation using in vivo assay. A total of thirty (30) adult male albino rats (130-170g) were divided into five (5) groups of six (6) rats each for the in-vivo anti-inflammatory assay. Group 1 normal; Group 2 was given 10 mg/kg body weight of Indomethacin and Groups 3, 4 and 5 were given 100, 200 and 400 mg/kg body weight of MERVL) respectively. Acute toxicity was carried out using standard method.Place and Duration of the Study: Department of Pharmacology Lab, Enugu State of University of Science and Technology Agbani Nigeria, between March 2021 and August 2021.Results: The percentage yield of the extract was 10.8%. The result of the quantitative and qualitative phytochemical screening of methanol extract of R. vomitoria leaves showed that Phenol (1590 � 55.66) Flavonoid (1168 � 11.43) and Tannin (1466 � 40.73) were present in high concentration whereas, Alkaloid (549 � 4.21) Glycoside (315 � 61.38) and Terpenoid (162 � 1.38) were present in moderate concentration. Saponin (1.16 � 0.23) and Steroid (0.84 � 0.03) were present in low concentrations. Acute toxicity tests showed no toxicity and mortality at doses up to 5000 mgkg-1. A significant (P < 0.05) reduction in the mean paw oedema was observed for all the treatment groups from 1 hour to 12 hours when compared to the normal group. The result shown that groups treated with 100, 200 and 400 mg/kg b.w of extract inhibit the inflammation in a non-dose dependent manner with percentage mobilization of 5.7%, 14.1% and 6.2% respectively.Conclusion: The study's results demonstrate that MERVL exhibits remarkable anti-inflammatory and anti-oxidant activities and the extract yielded a better reduction in edema and increases mobilization in leukocyte in response to agar suspension. It also demonstrates that MERVL has a modulatory effect on the vascular changes that accompany inflammation. The plant's anti-inflammatory properties could potentially result from the interaction of different photochemical substances present. The findings suggest that, if used, the plant may serve as a source of anti-inflammatory agents.en_US
dc.identifier.affiliationsDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeria and Department of Biochemistry, Faculty of Biological Science, University of Nigeria, Nsukka, Nigeriaen_US
dc.identifier.affiliationsDepartment of Biochemistry, Faculty of Natural Sciences, Micheal Okpara University of Agriculture Umudike Abia State, Nigeriaen_US
dc.identifier.affiliationsDepartment of Medical Biochemistry, College of Medicine, Esut, Parklane, Nigeriaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeriaen_US
dc.identifier.affiliationsDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeriaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeriaen_US
dc.identifier.affiliationsDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeriaen_US
dc.identifier.affiliationsDepartment of Biochemistry, Faculty of Natural and Applied Sciences, State University of Medical and Applied Sciences, Igbo Eno. Enugu State, Nigeriaen_US
dc.identifier.affiliationsDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Agbani, Nigeriaen_US
dc.identifier.affiliationsDepartment of Biochemistry, Faculty of Biological Science, University of Nigeria, Nsukka, Nigeriaen_US
dc.identifier.citationCelestine Ugwu Obiora, Jacinta Ukpabi-Ugo Chigozie, Lilian Asadu Chidinma, Jude Ali Ibeabuchi, Keneolisa Asogwa Felix, Walter Ugodi Gerald, Emmanuel Obasi Uchenna, Gloria Ogbonna Chidera, Kingsley Nwokedi Chukwuebuka, Assumpta Anosike Chioma. Evaluation of the Anti-inflammatory Effects of Methanol Extract of Rauwofia vomitera Leaves (MERVL) Using In-vivo Based Assays . International Journal of Medical Research & Health Sciences. 2024-May; 33(5): 70-78en_US
dc.identifier.issn2231-086X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/226840
dc.languageenen_US
dc.publisherMs. M. B. Mondalen_US
dc.relation.issuenumber5en_US
dc.relation.volume33en_US
dc.source.urihttps://doi.org/10.9734/ijbcrr/2024/v33i5877en_US
dc.subjectRauwofia VOMITERAen_US
dc.subjectegg albuminen_US
dc.subjectanti-inflammatoryen_US
dc.subjectleucocyte mobilizationen_US
dc.subjectmethanol extracten_US
dc.titleEvaluation of the Anti-inflammatory Effects of Methanol Extract of Rauwofia vomitera Leaves (MERVL) Using In-vivo Based Assaysen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ijbcrr2024v33n5p70.pdf
Size:
224.31 KB
Format:
Adobe Portable Document Format