Recombinant secretory proteins based new ‘Cocktail ELISA’ as a marker assay to differentiate infected and vaccinated cows for Mycobacterium avium subspecies paratuberculosis infection

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dc.contributor.authorChaubey, Kundan Kumaren_US
dc.contributor.authorSingh, Shoor Viren_US
dc.contributor.authorBhatia, Ashok Kumaren_US
dc.date.accessioned2020-01-02T06:38:39Z
dc.date.available2020-01-02T06:38:39Z
dc.date.issued2019-12
dc.description.abstractJohne's disease is endemic in the domestic livestock population of India. Recently, we developed highly effective 'Indigenous vaccine' to control Johne’s disease in animals. In order to gain disease free status as per World Organization for Animal Health, it is essential to have a marker assay to differentiate between infected and vaccinated animals before vaccine can be used in the field. We have developed a new marker assay ‘Cocktail ELISA’ using six ‘recombinant secretary proteins’ (MAP 1693c, MAP 2168c, MAP Mod D, MAP 85c, MAP Pep AN and MAP Pep AC) and evaluated for diagnosis of Johne’s disease along with 'Indigenous ELISA kit'. This ‘Cocktail ELISA’ successfully differentiated the infected, vaccinated and healthy (non-infected) cows and will facilitate the use of Johne’s disease vaccine to control the disease in cows at national levels.en_US
dc.identifier.affiliationsDepartment of Biotechnology, GLA University, Chaumuhan, Mathura, Uttar Pradesh-281 406, Indiaen_US
dc.identifier.citationChaubey Kundan Kumar, Singh Shoor Vir, Bhatia Ashok Kumar. . Indian Journal of Experimental Biology. 2019 Dec; 57(12):en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/191430
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber12en_US
dc.relation.volume57en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/52195en_US
dc.titleRecombinant secretory proteins based new ‘Cocktail ELISA’ as a marker assay to differentiate infected and vaccinated cows for Mycobacterium avium subspecies paratuberculosis infectionen_US
dc.typeJournal Articleen_US
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