Statistical medium optimization of an alkaline protease from Pseudomonas aeruginosa MTCC 10501, its characterization and application in leather processing.

Simple item page
dc.contributor.authorBoopathy, Naidu Ramachandra
dc.contributor.authorIndhuja, Devadas
dc.contributor.authorSrinivasan, Krishnan
dc.contributor.authorUthirappan, Mani
dc.contributor.authorGupta, Rishikesh
dc.contributor.authorRamudu, Kamini Numbi
dc.contributor.authorChellan, Rose
dc.date.accessioned2013-07-31T04:38:23Z
dc.date.available2013-07-31T04:38:23Z
dc.date.issued2013-04
dc.description.abstractProteases are shown to have greener mode of application in leather processing for dehairing of goat skins and cow hides. Production of protease by submerged fermentation with potent activity is reported using a new isolate P. aeruginosa MTCC 10501. The production parameters were optimized by statistical methods such as Plackett-Burman and response surface methodology. The optimized production medium contained (g/L); tryptone, 2.5; yeast extract, 3.0; skim milk 30.0; dextrose 1.0; inoculum concentration 4%: initial pH 6.0; incubation temperature 30 °C and optimum production at 48 h with protease activity of 7.6 U/mL. The protease had the following characteristics: pH optima, 9.0; temperature optima 50 °C; pH stability between 5.0-10.0 and temperature stability between 10-40 °C. The protease was observed to have high potential for dehairing of goat skins in the pre- tanning process comparable to that of the chemical process as evidenced by histology. The method offers cleaner processing using enzyme only instead of toxic chemicals in the pre-tanning process of leather manufacture.en_US
dc.identifier.citationBoopathy Naidu Ramachandra, Indhuja Devadas, Srinivasan Krishnan, Uthirappan Mani, Gupta Rishikesh, Ramudu Kamini Numbi, Chellan Rose. Statistical medium optimization of an alkaline protease from Pseudomonas aeruginosa MTCC 10501, its characterization and application in leather processing. Indian Journal of Experimental Biology. 2013 Apr; 51(4): 336-342.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/147599
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/16555en_US
dc.subjectDehairingen_US
dc.subjectProteaseen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectResponse surface methodologyen_US
dc.subject.meshAnimals
dc.subject.meshBacterial Proteins --biosynthesis
dc.subject.meshCulture Media
dc.subject.meshEndopeptidases --biosynthesis
dc.subject.meshGoats
dc.subject.meshHydrogen-Ion Concentration
dc.subject.meshIndustrial Microbiology --methods
dc.subject.meshIndustry
dc.subject.meshModels, Statistical
dc.subject.meshPeptones --chemistry
dc.subject.meshPressure
dc.subject.meshPseudomonas aeruginosa --metabolism
dc.subject.meshTanning
dc.subject.meshTemperature
dc.subject.meshYeasts --chemistry
dc.titleStatistical medium optimization of an alkaline protease from Pseudomonas aeruginosa MTCC 10501, its characterization and application in leather processing.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2013v51n4p336.pdf
Size:
232.62 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology