Protective Role of Onion Oil on Hepato- testicular Oxidative Damage Induced by Gamma Irradiation in Rats.
Loading...
Date
2014-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aim: Onions have potential antioxidant properties that lower the oxidative status. The
Present study was aimed to investigate the antioxidant activity of onion oil in irradiated
male albino rats.
Study Design: Randomized controlled experiment.
Place and Duration of Study: Experimental Animal Unit, Drug Radiation Research
Department, National Center for Radiation Research and Technology, Cairo Egypt.
Methodology: Animals were divided to the following groups each of eight rats. Control
group, Onion oil group: Rats received 200 mg/kg b.wt onion oil orally for seven days. IRR
group: Rats were exposed to γ- ray as a fractionated dose of 9 Gy for 7 days, Onion oil +
IRR group: Rats were administered with onion oil orally along with γ- ray exposure. At the
end of this study the animals were sacrificed and the effects of onion oil against hepatotesticular
oxidative damages were monitored by assaying the levels of serum alanine
transferase (ALT), aspartate aminotransferase (AST), alkaline phoshatase (ALP), as well
as testosterone and DHEA level. In addition both liver and testis lipid peroxidation (MDA),
superoxide dismutase (SOD), and catalase (CAT) were examined.
Results: The results showed a significant increase in serum ACP, AST, and ALT
activities (107.7%, 91.1% and 100.2% compared to control) with a decrease in
testosterone (12% compared to control) and non- significant change in DHEA. The levels
of SOD (59.31±10.67 & 0.21± 0.022), CAT (0.2081± 0.022 & 0.247±0.0453) and GSH
(0.32±0.046 & 3.46± 0.476) were significantly decreased in irradiated group accompanied by significant increase in both liver and testis MDA (171.2±13.69
202.2±49.34) respectively. The levels of reversal effects of irradiation were shown by
Onion oil + IRR group in both liver and testis.
Conclusion: Data concluded that onion oil showed the reversal effects of ionizing
radiation induced hepato- testicular oxidative stress.
Description
Keywords
Onion oil, Gamma Irradiation, DHEA, Testosterone, Antioxidant Activity
Citation
Gharib O A. Protective Role of Onion Oil on Hepato- testicular Oxidative Damage Induced by Gamma Irradiation in Rats. European Journal of Medicinal Plants. 2014 Feb; 4(2): 135-144.