Ameliorative Effect of Quercetin and Luteolin Supplements on Histology of Liver and Lungs Intoxicated with Nicotine in Young Rats
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Date
2019-02
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Pharmaceutical Research and Allied Sciences
Abstract
Background: Cyclosporin A (CsA) is immunosuppressive drug, but nephrotoxicity has been a major limiting factor. Thepresent study aimed to evaluate the protective effect of parsley leaves extract and silymarin against nephrotoxicity inducedby Cyclosporin A in rats. Methods: sixty male albino rats divided into six groups (n=10). Group I control group. Group IIreceived single dose of CsA (50mg/kg weight, orally/day). Group III received parsley leaves extract (250mg/kg b.wtorally/day). Group IV received CsA and parsley leaves extract as in group II and III. Group V silymarin group receivedsilymarin (100 mg/kg b.wt orally/day). Group VI received CsA and silymarin as in group II and V. Serum urea, uric acidand Creatinine were measured. Biomarkers of oxidative stress, antioxidant status, DNA damage, apoptosis andinflammatory-mediators had been measured in kidneys tissues. Results: Administration of Cyclosporin A to rats inducednephrotoxicity associated with a significant increase in serum urea, uric acid and Creatinine. Significant increase in MDA,NO, 8-OHdG, caspase-3, NF-κB, TNF-α and significant decrease of GST in kidney tissues. Histopathological examination ofanimal treated with CsA exhibited disruption of normal kidney architecture; cellular disorganization, intracellular vacuoles,and formation of many inflammatory cells. Co-administration of parsley leaves extract or silymaren+ CsA attenuated all ofthe parameters near to the normal. However, pronounced attenuation was recorded in animals co treated with parsleyleaves extract. Conclusion: The study suggested that the anti-oxidant and anti-inflammatory effect of parsley leaves extractmay prevent CsA-induced nephrotoxicity via decreasing the oxidative stress, and repairing the histopathological changes.
Description
Keywords
Nicotine, quercetin, luteolin, toxicity, supplements.
Citation
Bafageeh Wejdan A., Abdelaziza Sahar A.. Ameliorative Effect of Quercetin and Luteolin Supplements on Histology of Liver and Lungs Intoxicated with Nicotine in Young Rats. International Journal of Medical Research Professionals. 2019 Apr; 8(2): 129-136