PROTECTIVE EFFECT OF BIFIDOBACTERIUM LONGUM SUSPENSION FORMULATED WITH MEDIUM CHAIN TRIGLYCERIDES OIL IN DNBS-INDUCED COLITIS IN SPRAGUE-DAWLEY RATS

Simple item page
dc.contributor.authorKheni, DBen_US
dc.contributor.authorSureja, VPen_US
dc.contributor.authorDeshpande, SSen_US
dc.contributor.authorDubey, VPen_US
dc.contributor.authorKansagra, JJen_US
dc.contributor.authorBariya, AHen_US
dc.date.accessioned2025-08-13T08:58:30Z
dc.date.available2025-08-13T08:58:30Z
dc.date.issued2025-04
dc.description.abstractOne of the most common chronic inflammatory diseases that causes extensive damage to the gastrointestinal tract (GIT) is inflammatory bowel disease (IBD). IBD symptoms can range in severity from mild and frequent diarrhea, bloating, and abdominal pain to severe bloody stools, anemia, unconsciousness, and occasionally even death. One of the main contributing factors to the pathophysiology of IBD is gut dysbiosis. The purpose of the current study was to determine how well a probiotic (Bifidobacterium longum) solution in medium-chain triglycerides (MCT) oil protected rats from colitis caused by dinitrobenzene sulfonic acid (DNBS). Sprague Dawley (SD) rats were given DNBS (120 mg/kg/rat) intrarectally to induce colitis, which was then allowed to persist for three days. Rats were randomly assigned to receive either B. longum oil suspension (3 x 106 CFU/g/day orally) or dexamethasone (2 mg/kg/day orally) for 28 days in a row after colitis induction. Body weight, average intake of food and water, histological analysis, colon weight, intestinal inflammatory biomarkers (fecal calprotectin), antioxidant potential (glutathione (GSH) and superoxide dismutase (SOD)), and biomarkers of oxidative stress (nitric oxide (NO) and malondialdehyde (MDA)). The disease activity index (DAI) and colonic mucosal damage index (CMDI) scores were used to assess the extent of colonic damage. The statistical analysis was conducted using GraphPad Prism software, with a significance threshold of p < 0.05. Body weight and food and water intake were significantly reduced in the DNBS-induced colitis group, while it was prevented by dexamethasone and B. longum oil suspension. Similarly, the degree of colonic damage brought on by DNBS was considerably reduced by dexamethasone and B. longum oil suspension therapy. This impact was followed by considerable improvement in the antioxidant biomarker levels (GSH and SOD) and reduction in the oxidative stress and intestinal inflammation level. Dexamethasone treatment outperformed B. longum oil suspension therapy in every analyzed metric. The study's findings demonstrate the protective effects of B. longum oil suspension therapy and dexamethasone in a DNBS-induced experimental colitis model. This effect may be explained by the anti-inflammatory and antioxidant properties of the treatments.en_US
dc.identifier.affiliationsKadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat, Indiaen_US
dc.identifier.affiliationsKadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat, Indiaen_US
dc.identifier.affiliationsK.B. Institute of Pharmaceutical Education and Research, Affiliated to Kadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat, Indiaen_US
dc.identifier.affiliationsSundyota Numandis Probioceuticals Pvt. Ltd., Ahmedabad, Gujarat, Indiaen_US
dc.identifier.affiliationsSundyota Numandis Probioceuticals Pvt. Ltd., Ahmedabad, Gujarat, Indiaen_US
dc.identifier.affiliationsArihant School of Pharmacy & Bio-Research, Ahmedabad, Gujarat, Indiaen_US
dc.identifier.citationKheni DB, Sureja VP, Deshpande SS, Dubey VP, Kansagra JJ, Bariya AH. PROTECTIVE EFFECT OF BIFIDOBACTERIUM LONGUM SUSPENSION FORMULATED WITH MEDIUM CHAIN TRIGLYCERIDES OIL IN DNBS-INDUCED COLITIS IN SPRAGUE-DAWLEY RATS. International Journal of Pharmaceutical Sciences and Drug Research . 2025 Apr; 17(2): 194-202en_US
dc.identifier.issn0975 – 248X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/250338
dc.languageenen_US
dc.publisherMRI Publication Pvt. Ltd.en_US
dc.relation.issuenumber2en_US
dc.relation.volume17en_US
dc.source.urihttps://doi.org/10.25004/IJPSDR.2025.170210en_US
dc.subjectProbioticen_US
dc.subjectBifidobacterium longumen_US
dc.subjectDexamethasoneen_US
dc.subjectInflammatory bowel diseaseen_US
dc.subjectOxidative stressen_US
dc.subjectInflammationen_US
dc.subjectGut dysbiosisen_US
dc.titlePROTECTIVE EFFECT OF BIFIDOBACTERIUM LONGUM SUSPENSION FORMULATED WITH MEDIUM CHAIN TRIGLYCERIDES OIL IN DNBS-INDUCED COLITIS IN SPRAGUE-DAWLEY RATSen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ijpsdr2025v17n2p194.pdf
Size:
931.17 KB
Format:
Adobe Portable Document Format
Download
Collections
International Journal of Pharmaceutical Sciences and Drug Research