The Clinical Significance Of Rassf1a And Cdh1 Hypermethylation In Breast Cancer Patients
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Date
2018-01
Journal Title
Journal ISSN
Volume Title
Publisher
World Wide Journals
Abstract
Introduction:Aberrant DNAmethylation patterns in serum DNAmight be used as a biomarker for the early diagnosis and management of cancer patients. The aim of present study was to evaluate DNAmethylation of RASSF1Aand CDH1 in circulating cell free DNA(cfDNA) from serum and paired tissue DNAsamples of breast cancer patients. Material and methods: Methylation-specific PCR was used to assess the methylation status of the two genes in serum and paired tissue sample DNAof 50 breast cancer patients. Biochemical parameters were assessed using an electrochemiluminescence analyzer. Results: Significant correlation found between methylation status of RASSF1A and CDH1 in serum and paired tissue samples of patients. Among clinicopathological findings, CDH1 methylation showed significant association with advance staging and tumor and methylation of RASSF1A exhibited significant association with progesterone receptor and estrogen receptor status in both serum and paired tissue. Vitamins levels were significantly high in cases compared to control group. High folic acid levels were significantly associated with the RASSF1Amethylation. Conclusions: These findings suggest that methylation of cfDNAmay be important in the early detection of breast cancer.
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Keywords
Methylation, RASSF1A, CDH1, Methylation-specific PCR, Vitamin B12, Folic acid
Citation
Nandi Kajal, Yadav Prasant, Mir Rashid, Khurana Nita, Agarwal PN, Saxena Alpana. A Study Of Ocular Manifestations In Autoimmune Connective Tissue Diseases In Adults. International Journal of Scientific Research. 2018 Jan; 7(1): 46-49