Development And Validation Of Rp-Hplc Method For Simultaneous Estimation Of Gallic Acid, Curcumin And Piperine In An Ayurvedic Formulation
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Date
2020-06
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Innovare Academic Sciences Pvt. Ltd.
Abstract
Objective: The objective of the present work was to establish a simple, precise, accurate and robust method for simultaneous estimation of gallic acid, curcumin and piperine from the marketed ayurvedic formulation by liquid chromatography.
Methods: The separation was carried out on Hemochrom C18 Column (250 mm × 4.6 mm ID, 5 µm pore size) with a mobile phase methanol: acetonitrile: water (pH 3.2adjusted by using orthophosphate acid) in the ratio 70:20:10v/v by isocratic elution modeat 25 °C and the flow rate was setat0.8 ml/min. The analysis was carried out atisoabsorptive wavelength of 295 nm.
Results: The retention time of gallic acid, curcumin and piperine was found to be 3.3(±0.2), 4.7 (±0.2) and 5.6 (±0.2) min, respectively. The linearity range for gallic acid, curcumin and piperine was found to be 10-70 μg/ml, 20-80 µg/ml and 2-14 µg/ml, respectively with the coefficient of linear regression greater than 0.99 for all markers. Mean percent recoveries for gallic acid, curcumin, and piperine were found within the limit of acceptance (99-100%). The percent relative standard deviation (%RSD) for precision and robustness was found less than 2%, which indicates the method is precise and robust. The developed method applied for quantification of these markers from the marketed ayurvedic formulation of Dekofcyn tablet.
Conclusion: The developed method was found to be simple, rapid, precise and reproducible for standardization of Dekofcyn tablet and can be useful for other formulations containing these three markers.
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Keywords
Reverse-phase high-performance liquid chromatography (RP-HPLC), Gallic acid, Curcumin, Piperine, Method validation, Ayurvedic formulation, Standardization
Citation
Shaikh M., Jadhav A. P.. Development And Validation Of Rp-Hplc Method For Simultaneous Estimation Of Gallic Acid, Curcumin And Piperine In An Ayurvedic Formulation. International Journal of Pharmacy and Pharmaceutical Sciences. 2020 Jun; 12(6): 76-80