Hormone-mediated positive and negative regulation of a silencer region containing laminin B2 promoter in astrocytes.

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dc.contributor.authorKedar, Ven_US
dc.contributor.authorFreeze, Een_US
dc.contributor.authorHempel, Fen_US
dc.date.accessioned2009-05-28T12:32:37Z
dc.date.available2009-05-28T12:32:37Z
dc.date.issued1996-10-01en_US
dc.description.abstractPrimary astrocytes synthesize only B2 chain (200 Kda) of laminin, which is one of the major components of basement membranes in the central nervous system (CNS). In CNS, B2 laminin functions as a potent neurite growth factor. Laminin B2 promoter contain no TATA or CAAT boxes but is GC rich. By deletion analysis and transient transfection assays of B2 laminin promoter, we have identified a silencer-like activity in the upstream region of the promoter. Thyroid hormone, insulin and phorbol ester mediated the induction of the promoter activity. Induction was repressed by the treatment of astrocytes with synthetic glucocorticoid hormone, dexamethasone. Hormone-mediated regulation through specific positive and negative responsive elements in transactivation of this silencer-containing TATA-less laminin B2 gene has been postulated.en_US
dc.description.affiliationLaboratory of Molecular Biology, National Institute of Neurological Disorder and Strokes, National Institute of Health, Bethesda, MD 20892, USA.en_US
dc.identifier.citationKedar V, Freeze E, Hempel F. Hormone-mediated positive and negative regulation of a silencer region containing laminin B2 promoter in astrocytes. Indian Journal of Experimental Biology. 1996 Oct; 34(10): 939-44en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58225
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAstrocytes --drug effectsen_US
dc.subject.meshGenes, Regulator --drug effectsen_US
dc.subject.meshHormones --pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLaminin --geneticsen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRatsen_US
dc.subject.meshTATA Boxen_US
dc.subject.meshTranscriptional Activation --drug effectsen_US
dc.titleHormone-mediated positive and negative regulation of a silencer region containing laminin B2 promoter in astrocytes.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, U.S. Gov't, P.H.S.en_US
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