Evaluating antimicrobial potential of ozonated water in preliminary trials

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dc.contributor.authorBaferaj, Mohammed Abdulhakim Oen_US
dc.contributor.authorMathew, Merinen_US
dc.contributor.authorGanji, Kiran Kumaren_US
dc.contributor.authorSelim, Samyen_US
dc.contributor.authorElamir, Mohammed Yagoub Mohammeden_US
dc.contributor.authorV, Rajeswarien_US
dc.date.accessioned2025-06-18T11:43:10Z
dc.date.available2025-06-18T11:43:10Z
dc.date.issued2025-05
dc.description.abstractIn response to the growing demand for sustainable disinfection solutions, ozonated water emerges as a promising antimicrobial agent. This study evaluates the efficacy of ozonated water against microbial strains such as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans under standardized laboratory conditions. Sterile distilled water was ozonated using a portable ozone generator of 13 W for 30 min. Ozone concentration was confirmed using test strips. Nutrient broth and peptone water were used for bacterial and fungal growth, respectively. The study had three groups: negative control (uninoculated media), positive control (media inoculated with microbes) and test group (ozonated media inoculated with microbes). Microbial growth was assessed by solution turbidity after 24 h for bacterial species and 48 h for fungi. Additionally, the bactericidal and fungicidal activities were determined by transferring 10 mL of the test group sample into fresh nutrient broth and peptone water, followed by incubation. The results confirmed antimicrobial activity of ozonated water. Also, the bactericidal and fungicidal activity of ozonated group confirmed by the clear broth in the test group, like the negative control, suggests its applications in various domains including healthcare. Further research is necessary to evaluate its compatibility with various materials for optimal applications.en_US
dc.identifier.affiliationsDepartment of Prosthetic Dental Sciences, Al Jouf, Saudi Arabiaen_US
dc.identifier.affiliationsDepartment of Prosthetic Dental Sciences, Al Jouf, Saudi Arabiaen_US
dc.identifier.affiliationsDepartment of Preventive Dental Sciences, College of Dentistry,Jouf University, Al Jouf, Saudi Arabiaen_US
dc.identifier.affiliationsDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Al Jouf, Saudi Arabiaen_US
dc.identifier.affiliationsDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Al Jouf, Saudi Arabiaen_US
dc.identifier.affiliationsDepartment of Microbiology, Viswabharathi Medical college and general hospital, Kurnool, Andhrapradesh, Indiaen_US
dc.identifier.citationBaferaj Mohammed Abdulhakim O, Mathew Merin, Ganji Kiran Kumar, Selim Samy, Elamir Mohammed Yagoub Mohammed, V Rajeswari. Evaluating antimicrobial potential of ozonated water in preliminary trials . Indian Journal of Experimental Biology. 2025 May; 63(5): 443-448en_US
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/249444
dc.languageenen_US
dc.publisherThe CSIR-National Institute of Science Communication and Policy Research (CSIR-NIScPR) (formerly known as NISCAIR).en_US
dc.relation.issuenumber5en_US
dc.relation.volume63en_US
dc.source.urihttps://doi.org/10.56042/ijeb.v63i05.15119en_US
dc.subjectCandida albicansen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectStaphylococcus aureusen_US
dc.titleEvaluating antimicrobial potential of ozonated water in preliminary trialsen_US
dc.typeJournal Articleen_US
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