Ameliorative Effects of the Methanolic Extract of the Rind of Citrullus lanatus on Lead Acetate Induced Toxicity on Semen Parameters and Reproductive Hormones of Male Albino Wistar Rats.
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2014-09
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Abstract
Aims: The present study investigated the effects of the methanolic extract of the rind of
Citrullus lanatus on lead acetate induced toxicity on semen parameters, reproductive
hormone assay and testicular histology in male albino Wistar rats.
Study Design: Controlled experimental study using randomly assigned laboratory
animals.
Place and Duration of Study: Department of Human Physiology, College of Health
Sciences, University of Port Harcourt, PMB 5323, Port Harcourt, Nigeria and Department
of Physiology, Madonna University, Elele Campus, Rivers State, Nigeria between January
2013 and February 2014.
Methodology: Twenty male rats were assigned into four groups: Group A to D of five rats
each. Group A served as control and received 2ml/kg bw of 10% extract vehicle; Group B received 200mg/kg bw of the methanolic extract of the rind of Citrullus lanatus; Group C
received 2.25mg/kg bw of lead acetate; and Group Dwere co-administered with 2.25
mg/kg bw lead acetate and 200 mg/kg bw of the methanolic extract of the rind of Citrullus
lanatus. The drugs and extracts were administered orally to the rats for 35days. On day
36, blood samples were collected from anaesthetized rats by cardiac puncture for
reproductive hormone assay and the testes harvested for determination of semen
parameters and histological studies. Semen parameters: count, motility, viability, and
morphology were determined and assay for plasma levels of Follicle Stimulating Hormone
(FSH), Luteinizing Hormone (LH) and testosterone done.
Results: Results obtained showed that, compared to control rats, administration of the
methanolic extract of the rind Citrullus lanatus significantly enhanced sperm count and all
reproductive hormone levels (P<0.05); and also caused non-significant increases in
sperm motility, percentage of spermatocytes with normal morphology and percentage of
live spermatocytes, but decreased percentage of dead spermatocytes (P>0.05).
Treatment with lead acetate caused a significant reduction in levels of all reproductive
hormones and significant diminution of sperm motility, morphology, viability; with
increases in percentage of dead spermatocytes (P<0.05). Expectedly, co-administration
of the methanolic extract of the rind of Citrullus lanatus with lead acetate ameliorated the
deleterious effects of lead acetate resulting in significant increases in sperm count and all
reproductive hormones (P<0.05) and non-significant increases in motility, morphology and
live spermatocytes (P>0.05): however, the percentage of spermatocytes with abnormal
heads were significantly increased. The results suggest that the methanolic extract of the
rind of Citrullus lanatus exerts a possible ameliorative effect on lead acetate induced
toxicity on some reproductive parameters in male albino Wistar rats.
Conclusion: The findings suggest that the methanolic extract of the rind of Citrullus
lanatus exerts a possible beneficial effect on male reproductive parameters in albino
Wistar rats and validates anecdotal reports of the beneficial effect of watermelon
consumption from our environment. We however, recommend further studies in this
regard.
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Kolawole T A, Dapper D V, Ojeka S O. Ameliorative Effects of the Methanolic Extract of the Rind of Citrullus lanatus on Lead Acetate Induced Toxicity on Semen Parameters and Reproductive Hormones of Male Albino Wistar Rats. European Journal of Medicinal Plants. 2014 Sept; 4(9): 1125-1137.