Anticancer activity of HMGA1 promoter targeting triplex forming oligonucleotide in HeLa cell line

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dc.contributor.authorAkhter, Md. Zahiden_US
dc.contributor.authorRajeswari, Moganty Ren_US
dc.date.accessioned2020-01-02T06:32:13Z
dc.date.available2020-01-02T06:32:13Z
dc.date.issued2018-04
dc.description.abstractHigh mobility group protein A1 (HMGA1) acts as an architectural transcription factor and regulates transcription of various genes. Upregulation of HMGA1 has been described in a large number of human malignancies and serves as a ‘tumor marker’. Due to its role in neoplastic transformation and tumor progression, hmga1 is considered as a promising therapeutic target. In the present study, we investigated the interaction of triplex forming oligonucleotide (TFO) of 18 bps targeted to hmga1 promoter (-1917 to -1940) and its influence on the expression of HMGA1 in HeLa cells. Stability of DNA triplex was characterized using various biophysical and thermodynamic methods and was confirmed by gel retardation assay using γ-32P [ATP]. Treatment of HeLa cells with hmga1 specific TFO significantly downregulated HMGA1 expression at mRNA, protein levels (~48%) and inhibited cell proliferation as investigated by RT-PCR, Western blot and Flow cytometry. The findings of the study suggest that TFO-mediated inhibition of hmga1 expression can be a promising strategy for modulation of gene expression and for inhibition of cancer cell proliferation. Moreover, DNA triplex-based therapeutic approaches hold promise in combating cancers associated with HMGA1 overexpression.en_US
dc.identifier.affiliationsDepartment of Biochemistry, All India Institute of Medical Sciences, New Delhi-110 029, Indiaen_US
dc.identifier.citationAkhter Md. Zahid, Rajeswari Moganty R. Biochemical assessment of extract from Oxalis corniculata L.: Its role in food preservation, antimicrobial and antioxidative paradigms using in situ and in vitro models. Indian Journal of Experimental Biology. 2018 Apr; 56(4): 230-243en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/190931
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber4en_US
dc.relation.volume56en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/44191en_US
dc.titleAnticancer activity of HMGA1 promoter targeting triplex forming oligonucleotide in HeLa cell lineen_US
dc.typeJournal Articleen_US
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