Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli.
| dc.contributor.author | Sheela, P | en_US |
| dc.contributor.author | Amuthan, G | en_US |
| dc.contributor.author | Mahadevan, A | en_US |
| dc.date.accessioned | 2009-05-28T13:56:52Z | |
| dc.date.available | 2009-05-28T13:56:52Z | |
| dc.date.issued | 1996-01-01 | en_US |
| dc.description.abstract | A lipase gene from X. campestris pv. sesami (strain XcS 1) causal agent of leaf spot disease of Sesamum indicum, was cloned onto E. colt. XcS showed the presence of lip+ transformants on Dye's medium with 1% glycerol as sole carbon source. The recombinant plasmids were isolated and when digested with Eco R1, yielded 2 fragments with molecular weights 4.0 and 4.8 kb. Thus a 8.8 kb insert DNA fragment was obtained which showed lipase activity. | en_US |
| dc.description.affiliation | Centre for Advanced Study in Botany, University of Madras. | en_US |
| dc.identifier.citation | Sheela P, Amuthan G, Mahadevan A. Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli. Indian Journal of Experimental Biology. 1996 Jan; 34(1): 27-31 | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/60072 | |
| dc.language.iso | eng | en_US |
| dc.source.uri | https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.asp | en_US |
| dc.subject.mesh | Cloning, Molecular | en_US |
| dc.subject.mesh | Escherichia coli --genetics | en_US |
| dc.subject.mesh | Genes, Bacterial | en_US |
| dc.subject.mesh | Lipase --genetics | en_US |
| dc.subject.mesh | Xanthomonas campestris --enzymology | en_US |
| dc.title | Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli. | en_US |
| dc.type | Journal Article | en_US |
| dc.type | Research Support, Non-U.S. Gov't | en_US |
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