Mini transposon vector mediated foreign gene expression in Mesorhizobium huakuii subsp. rengei.

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dc.contributor.authorBalachandar, Den_US
dc.contributor.authorKannaiyan, Sen_US
dc.contributor.authorOno, Hen_US
dc.contributor.authorMurooka, Yen_US
dc.date.accessioned2009-05-28T10:44:49Z
dc.date.available2009-05-28T10:44:49Z
dc.date.issued2004-10-30en_US
dc.description.abstractAmong the transposable elements, mini-Tn5 transposon vector has proven to be of greater utility for insertion mutagenesis of variety of Gram negative bacteria. The mini-Tn5 vector containing promoter less egfp gene and gentamycin resistant gene was used for the present study. The transposon vector was introduced to M. huakuii from E. coli S17 by conjugation. The conjugants were screened for stable expression of egfp both in free-living and in nodules of Astragalus sinicus. The result showed that the conjugant #3 showed stable expression of green fluorescent both in free-living and bacteroid stage. The visualization of sym plasmid of wild strain and conjugants showed that conjugant #3 had a fragmentation of large sized plasmid into two but without affecting the nodulating ability. These results clearly indicated that mini-Tn5 vectors (Transposon vectors) the best alternate tools for plasmid vectors for integration of foreign genes in chromosomal DNA or symbiotic plasmid and expression, both in free-living and bacteroid stage of Rhizobium.en_US
dc.description.affiliationDepartment of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India. dbalu2000@yahoo.comen_US
dc.identifier.citationBalachandar D, Kannaiyan S, Ono H, Murooka Y. Mini transposon vector mediated foreign gene expression in Mesorhizobium huakuii subsp. rengei. Indian Journal of Experimental Biology. 2004 Oct; 42(10): 1028-31en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/55740
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAlphaproteobacteria --geneticsen_US
dc.subject.meshAstragalus Plant --microbiologyen_US
dc.subject.meshConjugation, Geneticen_US
dc.subject.meshDNA Transposable Elements --geneticsen_US
dc.subject.meshEscherichia coli --geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGenetic Vectorsen_US
dc.subject.meshGreen Fluorescent Proteins --geneticsen_US
dc.subject.meshRecombinant Proteins --geneticsen_US
dc.titleMini transposon vector mediated foreign gene expression in Mesorhizobium huakuii subsp. rengei.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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