Effect of poly-D-lysine on proliferation and senescence of caprine spermatogonial stem cells in vitro

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dc.contributor.authorGupta, Deekshaen_US
dc.contributor.authorKharche, Suresh Dinkaren_US
dc.contributor.authorSingh, Shiva Pratapen_US
dc.contributor.authorPathak, Juhen_US
dc.date.accessioned2023-08-09T06:20:16Z
dc.date.available2023-08-09T06:20:16Z
dc.date.issued2022-03
dc.description.abstractSpermatogonial stem cells (SSCs) have the unique ability of both self-renewing and to produce progeny that undergoes differentiation to spermatozoa. As SSCs exist in very low numbers, therefore efficient in vitro expansion of SSCs is important prior to their clinical applications. In this study, we tried to improve the functionality of putative SSCs (pSSCs) during culture using poly-D-lysine (PDL) coating. For this, plates were coated with 0.01% PDL with different coating time interim treatments (5, 30 and 60 min) while control remained uncoated. The adequate amount of pSSCs of the goat was isolated and enriched using two-step enzymatic digestion and differential plating methods. Further, the functionality of pSSCs was evaluated by cell growth analysis, cell proliferation, senescence, and the presence of pluripotency (alkaline phosphatase, OCT-4) and SSC related (PGP-9.5) markers. The number and size of pSSCs colonies in 0.01% PDL coating groups were significantly (P <0.05) higher than in the control group. Similarly, pSSCs on uncoated plates expressed significantly (P <0.05) higheren_US
dc.identifier.affiliationsAnimal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281 122, Uttar Pradesh, Indiaen_US
dc.identifier.citationGupta Deeksha, Kharche Suresh Dinkar, Singh Shiva Pratap, Pathak Juh. Effect of poly-D-lysine on proliferation and senescence of caprine spermatogonial stem cells in vitro. Indian Journal of Experimental Biology. 2022 Mar; 60(3): 161-168en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/222466
dc.languageenen_US
dc.publisherCSIR-NIScPRen_US
dc.relation.issuenumber3en_US
dc.relation.volume60en_US
dc.source.urihttps://doi.org/10.56042/ijeb.v60i03.61049en_US
dc.subjectAgingen_US
dc.subjectGoaten_US
dc.subjectCapra hircusen_US
dc.titleEffect of poly-D-lysine on proliferation and senescence of caprine spermatogonial stem cells in vitroen_US
dc.typeJournal Articleen_US
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