Isolation, purification and immunological evaluation of toxin Hb from scorpion Heterometrus bengalensis (C.L. Koch) venom.

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dc.contributor.authorDasgupta, S Cen_US
dc.contributor.authorGomes, Aen_US
dc.contributor.authorGomes, Aen_US
dc.contributor.authorBasu, Aen_US
dc.contributor.authorLahiri, S Cen_US
dc.date.accessioned2009-05-28T11:29:28Z
dc.date.available2009-05-28T11:29:28Z
dc.date.issued1990-02-01en_US
dc.description.abstractToxin-Hb, a lethal toxic antigenic protein, isolated from the venom of H. bengalensis by CM-cellulose ion-exchange chromatography was a heat labile basic protein with a molecular weight of 10 kDa. It produced irreversible blockade on the isolated rat phrenic nerve diaphragm and chick biventer cervicis. LD50 of toxin Hb was 0.48 mg/kg (iv) in mice. Antiserum was raised in mice by hyperimmunization against toxin Hb. Antitoxin Hb antiserum was immunologically potent as revealed by immunogel-diffusion and immunoelectrophoresis. Five fold protection against the lethal action of toxin Hb was achieved by the antiserum. It also effectively antagonised toxin Hb induced neuromuscular blockade on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparations.en_US
dc.description.affiliationDepartment of Pharmacology, School of Tropical Medicine, Calcutta, India.en_US
dc.identifier.citationDasgupta SC, Gomes A, Gomes A, Basu A, Lahiri SC. Isolation, purification and immunological evaluation of toxin Hb from scorpion Heterometrus bengalensis (C.L. Koch) venom. Indian Journal of Experimental Biology. 1990 Feb; 28(2): 144-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56787
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChickensen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshImmune Seraen_US
dc.subject.meshMiceen_US
dc.subject.meshRatsen_US
dc.subject.meshScorpion Venoms --immunologyen_US
dc.subject.meshScorpionsen_US
dc.subject.meshTemperatureen_US
dc.titleIsolation, purification and immunological evaluation of toxin Hb from scorpion Heterometrus bengalensis (C.L. Koch) venom.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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