Transformation system of Beauveria bassiana and Metarhizium anisopliae using nitrate reductase gene of Aspergillus nidulans.

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dc.contributor.authorSandhu, S Sen_US
dc.contributor.authorKinghorn, J Ren_US
dc.contributor.authorRajak, R Cen_US
dc.contributor.authorUnkles, S Een_US
dc.date.accessioned2009-05-28T11:45:35Z
dc.date.available2009-05-28T11:45:35Z
dc.date.issued2001-07-22en_US
dc.description.abstractAn heterologous transformation system for entomopathogenic fungi B. bassiana and M. anisopliae was developed based on the use of A. nidulans nitrate reductase gene (niaD). B. bassiana and M. anisopliae niaD stable mutants were selected by treatment of protoplast with ethane methane sulphonate (EMS) and regenerated on chlorate medium. The cloned gene was capable of transforming B. bassiana and M. anisopliae at a frequency of 5.8 to 20 transformants per microg of DNA. Most of them were mitotically stable.en_US
dc.description.affiliationDepartment of Biological Science, Rani Durgawati University, Jabalpur, India.en_US
dc.identifier.citationSandhu SS, Kinghorn JR, Rajak RC, Unkles SE. Transformation system of Beauveria bassiana and Metarhizium anisopliae using nitrate reductase gene of Aspergillus nidulans. Indian Journal of Experimental Biology. 2001 Jul; 39(7): 650-3en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/57161
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAspergillus niger --enzymologyen_US
dc.subject.meshGenes, Fungalen_US
dc.subject.meshHypocreales --cytologyen_US
dc.subject.meshMitosisen_US
dc.subject.meshMutationen_US
dc.subject.meshNitrate Reductaseen_US
dc.subject.meshNitrate Reductases --geneticsen_US
dc.subject.meshTransformation, Geneticen_US
dc.titleTransformation system of Beauveria bassiana and Metarhizium anisopliae using nitrate reductase gene of Aspergillus nidulans.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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