In vitro multiple shoot regeneration and plant production in Alysicarpus rugosus DC. var. heyneanus Baker.

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dc.contributor.authorBhosle, S Ven_US
dc.contributor.authorThengane, R Jen_US
dc.contributor.authorThengane, S Ren_US
dc.date.accessioned2009-05-28T15:16:51Z
dc.date.available2009-05-28T15:16:51Z
dc.date.issued2005-10-21en_US
dc.description.abstractA protocol for in vitro multiple shoot regeneration and plant production through seedling (shoot tip) culture was established for Alysicarpus rugosus DC. var. heyneanus Baker. Maximum number of adventitious shoots (14.4) per shoot tip explant were initiated after two subcultures on MS solid medium supplemented with IAA (2.85 microM) plus BAP (2.22 microM) after 4 weeks. Shoot elongation (3.0-3.5 cm) was achieved on MS medium without any hormones. Stunted shoots elongated on half MS medium without growth hormones. Rooting occurred in MS medium containing IAA (1.14 - 2.85 microM) alone or in combination with IBA (0.89 - 2.46 microM) and or NAA (1.07 - 2.69 microM). Maximum rooting was established in MS medium supplemented with IAA (2.85 microM). The plants were acclimatized successfully with 55% survival in pot containing cocoa peat and sand (1:1). After a month, hardened plants were transferred to pots with manure, garden soil and sand (1:2:1) for further growth and finally planted in field.en_US
dc.description.affiliationDepartment of Botany, University of Pune, Pune 411007, India.en_US
dc.identifier.citationBhosle SV, Thengane RJ, Thengane SR. In vitro multiple shoot regeneration and plant production in Alysicarpus rugosus DC. var. heyneanus Baker. Indian Journal of Experimental Biology. 2005 Oct; 43(10): 916-20en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/61785
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFabaceae --metabolismen_US
dc.subject.meshPlant Growth Regulators --metabolismen_US
dc.subject.meshPlant Physiological Phenomenaen_US
dc.subject.meshPlant Rootsen_US
dc.subject.meshPlant Shoots --physiologyen_US
dc.subject.meshRegenerationen_US
dc.subject.meshTime Factorsen_US
dc.titleIn vitro multiple shoot regeneration and plant production in Alysicarpus rugosus DC. var. heyneanus Baker.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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