Relative ranking of culture media based on proliferation kinetics and spontaneous chromosomal aberrations in PHA-responsive human peripheral blood lymphocytes.

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dc.contributor.authorJha, A Nen_US
dc.date.accessioned2009-05-28T16:29:54Z
dc.date.available2009-05-28T16:29:54Z
dc.date.issued1992-08-01en_US
dc.description.abstractProliferation kinetics and spontaneous yield of chromosomal aberrations phytohemagglutinin (PHA)-responsive peripheral blood lymphocytes were studied from blood samples collected from 45 individuals in 4 different synthetic media. Except for a significant difference for Eagle's MEM and RPMI 1640, the other media did not show difference for the yield of chromatid or chromosome type of aberrations. Differences were however noticed in the proliferation kinetics (mitotic and proliferative rate indices) of cells among the media used. The study indicated that (i) the intrinsic properties of media which influence proliferation rate and yield of chromosomal aberrations are independent of each other as higher proportion of first division cells do not correspond with higher frequency of chromosomal aberrations, (ii) the amount of free-radical scavengers present in the medium, apart from the genetic make-up of the individuals, may contribute to the spontaneous yield of chromosomal aberrations and (iii) RPMI 1640 medium, which showed higher transformation and faster cycling rate for the lymphocytes, may be considered as medium of choice for analysing two main cytogenetic end-points, chromosomal aberrations and sister chromatid exchanges (SCEs).en_US
dc.description.affiliationCytogenetics Laboratory, Banaras Hindu University, Varanasi, India.en_US
dc.identifier.citationJha AN. Relative ranking of culture media based on proliferation kinetics and spontaneous chromosomal aberrations in PHA-responsive human peripheral blood lymphocytes. Indian Journal of Experimental Biology. 1992 Aug; 30(8): 751-3en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/63308
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshCell Division --drug effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChromosome Aberrationsen_US
dc.subject.meshChromosomes --drug effectsen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphocytes --ultrastructureen_US
dc.subject.meshMaleen_US
dc.subject.meshPhytohemagglutininsen_US
dc.titleRelative ranking of culture media based on proliferation kinetics and spontaneous chromosomal aberrations in PHA-responsive human peripheral blood lymphocytes.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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