Isolation and Identification of Xylanolytic Enzyme from an Effective Strain Bacillus Licheniformis Isolated from the Decaying Wood.
Loading...
Date
2013-10
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A new species of Bacillus licheniformis produced extracellular xylanase under submerged
fermentation when wheat bran is used as carbon source. The xylan is the most common hemicellulosic
polysaccharide in food industry and agricultural wastes, comprising a backbone of xylose residues linked by
β-1,4 glycosidic bonds. Bacillus licheniformis has been shown to be a promising organism for enhanced
production of xylanases & β-xylosidase under submerged fermentation (SmF). The optimization of cultural
conditions and carbon, nitrogen sources for enzymes production. The bacterial strain Bacillus licheniformis
was cultivated using as substrate xylan, wheat bran, corn straw, corncob, and sugarcane bagasse. Wheat bran
has been a good xylanase (16.8U/ml) & β xylosidase (5.6U/ml) activity after 48h of fermentation. Maximum
enzyme activity was observed in xylan as carbon source and peptone as nitrogen source. Both crude enzymes
were characterized and a bacterial xylanase shows optimum pH for xylanase activity at 6.5 & β xylosidase
were found to be 6.0. The optimum temperatures were 450C for both and they were thermally stable up to
500C. The parameters of Vmax and Km obtained using Line weaver-Burk plot method were 277.7μmol /
min/mg and 5.26 mg /L correspondingly.
Description
Keywords
Xylanase, β xylosidase, Bacillus licheniformis, Vmax and Km.
Citation
Chaturvedi Sarika, Singh Rajni, Khurana S M Paul. Isolation and Identification of Xylanolytic Enzyme from an Effective Strain Bacillus Licheniformis Isolated from the Decaying Wood. International Journal of Applied Biology and Pharmaceutical Technology. 2013 Oct-Dec; 4(4): 92-100.