‘Indigenous’ and ‘Ethanol Vortex’ ELISA kits for diagnosis of Mycobacterium avium subsp. paratuberculosis infection in cattle: Is there a ‘globally relevant kit’ in the ‘Reverse Ice-burg’ environment?

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dc.contributor.authorChaubey, Kundan Kumaren_US
dc.contributor.authorSingh, Shoor Viren_US
dc.contributor.authorBhatia, Ashok Kumaren_US
dc.date.accessioned2020-01-02T06:32:13Z
dc.date.available2020-01-02T06:32:13Z
dc.date.issued2018-04
dc.description.abstractHighly versatile and robust 'Indigenous ELISA kit' for the diagnosis of Mycobacterium avium subsp. paratuberculosis infection in cattle herds was compared with 'Ethanol Vortex (EV) ELISA kit' of USA. Of 160 (118 vaccinated and 42 non-vaccinated) cattle screened, 129 and 35 were positive in 'Indigenous' and 'Ethanol Vortex' ELISA kits, respectively. 'I-ELISA', using 'semi-purified protoplasmic antigen' from native highly prevalent biotype ('Indian Bison type') of MAP of goat origin, was highly sensitivitive (91.4%) as compared to the 'EV-ELISA. 'I-ELISA kit using whole cell sonicate from native 'S 5' (‘Indian Bison type’) strain of MAP as 'antigen source' was significantly superior than EV-ELISA kit using surface antigens from 'Linda' strain ('cattle type') of cattle origin in USA. Therefore, 'i-ELISA kit' may be recommended for the screening of domestic cattle herds against MAP infection in India. The present study has demonstrated that in the diagnosis of chronic infections and diseases, such as Johne's disease, 'Indigenous kits' are significantly superior to kits made in other countries, EV-ELISA, in the present case, particularly screening of native cattle herds endemically infected with MAP.en_US
dc.identifier.affiliationsMicrobiology Lab, Animal Health Division, ICAR-Central Institute for Research on Goats, Makhdoom, PO-Farah, Mathura, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDepartment of Microbiology and Immunology, GLA University, Mathura, Uttar Pradesh, Indiaen_US
dc.identifier.citationChaubey Kundan Kumar, Singh Shoor Vir, Bhatia Ashok Kumar. Estrogenized mouse model of polycystic ovary highlights mitochondrial pathway of apoptosis. Indian Journal of Experimental Biology. 2018 Apr; 56(4): 293-304en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/190939
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber4en_US
dc.relation.volume56en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/44184en_US
dc.title‘Indigenous’ and ‘Ethanol Vortex’ ELISA kits for diagnosis of Mycobacterium avium subsp. paratuberculosis infection in cattle: Is there a ‘globally relevant kit’ in the ‘Reverse Ice-burg’ environment?en_US
dc.typeJournal Articleen_US
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