Screening and characterization of high lipid accumulating microalga Ankistrodesmus sp. from freshwater environment

dc.contributor.authorRadha, Sen_US
dc.contributor.authorDharani, Renuka Sen_US
dc.contributor.authorDevi, Gayathri Sen_US
dc.contributor.authorRamya, Men_US
dc.date.accessioned2020-01-02T06:38:38Z
dc.date.available2020-01-02T06:38:38Z
dc.date.issued2019-12
dc.description.abstractMicroalgae based biofuel is an attractive alternative energy source due to its rapid growth rate and high lipid accumulation efficiency. In this study, we screened high lipid content microalga with the favourable fatty acid composition suitable for biodiesel production. Totally twelve different microalgal species were isolated from freshwater habitats. The isolates were identified as Micractinium sp., Chlorella sorokiniana, Scenedesmus bajacalifornicus, Desmodesmus sp., Scenedesmus obliquus, Coelastrum proboscideum, Chlamydomonas moewusii, Chlamydomonas debaryana, Chlamydomonas dorsoventralis, Coelastrum sp., and Ankistrodesmus sp. based on morphological features and ITS region similarity. Among the isolates, highest lipid content (33±0.07%) and lipid productivity (0.27±0.06 g L-1) were obtained from Ankistrodesmus sp. Intracellular lipid droplets of Ankistrodesmus sp. were observed through Nile red staining. The lipid content was enhanced up to 45% under the nitrogen deficient (5 mg L-1) BG-11 medium. Ankistrodesmus sp. fatty acid profile shows the presence of palmitic (16.39%), stearic (15.67%), oleic (25.66%), linolenic (21.62%), and alpha-linoleic acids (14.34%). The oleic acid was the dominant fatty acid 25.66% in the nitrogen deficient condition.en_US
dc.identifier.affiliationsDepartment of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, Indiaen_US
dc.identifier.citationRadha S, Dharani Renuka S, Devi Gayathri S, Ramya M. Isolation and molecular identification of α-amylase producing bacteria from hot water spring by 16S rRNA gene sequencing. Indian Journal of Experimental Biology. 2019 Dec; 57(12): 937-944en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/191424
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber12en_US
dc.relation.volume57en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/52201en_US
dc.titleScreening and characterization of high lipid accumulating microalga Ankistrodesmus sp. from freshwater environmenten_US
dc.typeJournal Articleen_US
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