Response Pattern of Antioxidants to Lipid Peroxide Concentration in Carbon Tetrachloride-Induced Hepato-Toxicity Is Tightly Logistic in Rabbits.
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Date
2011-10
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Abstract
Logistic response of antioxidants to lipid peroxide concentration in carbon tetrachloride
toxicity in rabbit liver was evaluated. Carbon tetrachloride (CCl4), ethanol extracts of
Chromolaena odorata (ETECO), sylimarin (a known hepatoprotective agent) and water,
were used to induce variations in the oxidant/antioxidant balance in the test and control
animals. This was used as a model to study the delicate balance between the activities
and/or the intracellular concentrations of these antioxidants and lipid peroxide.
Concentrations of lipid peroxidation product (malondialdehyde) were estimated to
access the degree of oxidation of the polyunsaturated fatty acids in the liver tissue.
Glutathione (GSH) concentration was estimated to capture the non-enzymatic
antioxidant concentration, while glutathione-s-transferase (GST), superoxide dismutase
(SOD), and catalase (CAT) activities were assayed in the liver to assess the enzymatic
antioxidant activities. Results obtained from this study showed that the concentrations of
lipid peroxidation product (malondialdehyde) varied in a logistic fashion with the nonenzymatic
antioxidant (glutathione) and the enzymatic antioxidants (glutathione-stransferase,
superoxide dismutase, and catalase). The concentration of the peroxidation
product and the concentration/activity of the antioxidants were inversely related,
maintaining a highly logistic relationship (R2 = 0.99). The non-enzymatic antioxidant
(GSH) concentration and the enzymatic antioxidant (GST, SOD, and CAT) activities
were found to be directly related in a sigmoidal manner (R2 = 0.98). These observations
indicated that oxidant/antioxidant concentrations and activities in a rabbit liver tissue is
tightly related and mathematically associated.
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Keywords
Toxicity, mathematical response, lipid peroxidation, oxidative stress, Chromolaena odorata
Citation
Alisi C S, Ojiako O A, Osuagwu C G, Onyeze G O C. Response Pattern of Antioxidants to Lipid Peroxide Concentration in Carbon Tetrachloride-Induced Hepato-Toxicity Is Tightly Logistic in Rabbits. European Journal of Medicinal Plants. 2011 Oct-Dec; 1(4): 118-129.