Abnormal erythrocyte membrane phospholipid organisation in chronic myeloid leukaemia.
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Date
1987-03
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Abstract
The membrane phospholipid organisation in the red cells of humans suffering
from chronic myeloid leukaemia has been analysed using the amino-group labelling reagent
trinitrobenzenesulphonic acid and the fluid-sensing fluorophore, Merocyanine 540. Unlike
the normal human erythrocytes, trinitrobenzenesulphonic acid in intact chronic myeloid
leukaemia erythrocytes modified about 30% phosphatidylserine, under controlled
conditions. Also, the chronic myeloid laukaemia red cells, but not the normal cells, were
found to bind the fluorescent dye Merocyanine 540. These results demonstrate that loss of
the transmembrane phospholipid asymmetry in chronic myeloid leukaemia erythrocytes is
accompanied by an enhancement in the outer surface fluidity and, therefore, suggest that the
red cells membrane phase-state asymmetry originates probably from the asymmetric arrangements
of phospholipids across the membrane bilayer.
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Keywords
Phospholipid asymmetry, erythrocytes, phase-state asymmetry, chronic myeloid leukaemia
Citation
Kumar A, Daniel S, Agarwal S S, Gupta C M. Abnormal erythrocyte membrane phospholipid organisation in chronic myeloid leukaemia. Journal of Biosciences. 1987 Mar; 11(1-4): 543-548.