Orlistat mitigates nitrosative stress and enhances paraoxonase-1 activity in serum and tissues of induced obese rats

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dc.contributor.authorKILINÇ, Sevtapen_US
dc.contributor.authorÖLÇÜO?LU, Rukiyeen_US
dc.contributor.authorY???T, Ay?e Arzuen_US
dc.date.accessioned2025-08-13T11:23:12Z
dc.date.available2025-08-13T11:23:12Z
dc.date.issued2025-06
dc.description.abstractThis study examined the effects of orlistat, an anti-obesity medication, on nitrosative stress and paraoxonase-1 (PON-1) enzyme activity in various tissues of rats with experimentally induced obesity. A total of 24 Wistar albino rats were divided into three groups: control, obese, and obese treated with orlistat (ORL). The ORL group received oral doses of 10 mg/kg/day for six weeks following an eight-week high-fat diet. During the study, body weights were monitored weekly, and various biochemical markers, including TNF-alpha, IL-1 beta, nitric oxide, nitrotyrosine, PON-1 enzyme activity, urea, creatinine, lactate dehydrogenase, and creatine kinase, were measured after the rats were sacrificed. The ORL group had significantly lower body weights compared to the obese group (P < 0.05). Obesity increased levels of inflammatory markers (TNF-alpha and nitric oxide) in serum, heart, and kidney tissues, as well as nitrotyrosine levels in serum, kidney, and testis tissues, compared to the control group (P < 0.05). Orlistat treatment reduced levels of TNF-alpha, IL-1 beta, nitric oxide, and nitrotyrosine in various tissues, as well as urea, creatinine, lactate dehydrogenase, and creatine kinase levels, compared to the obese group (P < 0.05). Moreover, orlistat significantly increased PON-1 enzyme activity in the heart, kidney, and testis tissues compared to the obese group (P < 0.05). These findings suggest that orlistat reduces inflammation and nitrosative stress and enhances antioxidant activity by increasing PON-1 enzyme levels. While orlistat shows promise as a therapeutic option for obesity, its systemic effects should be carefully considered.en_US
dc.identifier.affiliationsBaskent University, Faculty of Medicine, Department of Physiology, Ankara, Turkeyen_US
dc.identifier.affiliationsBaskent University, Faculty of Medicine, Department of Physiology, Ankara, Turkeyen_US
dc.identifier.affiliationsBaskent University, Faculty of Medicine, Department of Physiology, Ankara, Turkeyen_US
dc.identifier.citationKILINÇ Sevtap, ÖLÇÜO?LU Rukiye, Y???T Ay?e Arzu . Orlistat mitigates nitrosative stress and enhances paraoxonase-1 activity in serum and tissues of induced obese rats . Indian Journal of Experimental Biology. 2025 Jun; 63(6): 473-481en_US
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/253764
dc.languageenen_US
dc.publisherThe India Publications and Information Directorate (CSIR-NISCAIR)en_US
dc.relation.issuenumber6en_US
dc.relation.volume63en_US
dc.source.urihttps://doi.org/10.56042/ijeb.v63i06.13729en_US
dc.subjectOrlistaten_US
dc.subjectObesityen_US
dc.subjectNitrosative stressen_US
dc.subjectParaoxonase enzyme activityen_US
dc.titleOrlistat mitigates nitrosative stress and enhances paraoxonase-1 activity in serum and tissues of induced obese ratsen_US
dc.typeJournal Articleen_US
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