Detection of tuberculous IgG antibodies using Mycobacterium tuberculosis H37Ra, excretory secretory antigen and tuberculin-purified protein derivative.

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dc.contributor.authorKumar, Sen_US
dc.contributor.authorChenthamarakshan, Ven_US
dc.contributor.authorReddy, M Ven_US
dc.contributor.authorNarang, Pen_US
dc.contributor.authorGupta, O Pen_US
dc.contributor.authorHarinath, B Cen_US
dc.date.accessioned2009-05-28T10:41:53Z
dc.date.available2009-05-28T10:41:53Z
dc.date.issued1994-03-01en_US
dc.description.abstractDifferent antigen preparations, viz. excretory-secretory antigen (ES Ag), phosphate buffer saline soluble antigen (PBS-S Ag) and sodium dodecyl sulphate soluble antigen (SDS-S Ag) of M. tuberculosis (M.tb) H37Ra strain along with tuberculin purified protein derivative (PPD) were employed in stick indirect ELISA to detect IgG antibodies in sera of sputum positive pulmonary tuberculosis cases. Sera from healthy individuals and individuals with diseases other than tuberculosis (cross-reacting diseases) were used as controls. ES antigen and PPD showed higher antibody titres in tuberculosis cases (GMT-1378 each) compared to PBS-S Ag (GMT-454) and SDS-S Ag (GMT-974). Thereafter, an extensive study was done analysing higher number of sera in each group for the detection of tuberculous IgG antibodies using ES Ag and PPD. The ES Ag showed better sensitivity (87%) and specificity (85%) compared to the sensitivity (73%) and specificity (78%) achieved with PPD. The ES Ag also showed higher IgG antibody titre (GMT-1068) than PPD (GMT-721). From the present study it can be envisaged that ES Ag has high diagnostic potential in tuberculosis.en_US
dc.description.affiliationDepartment of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India.en_US
dc.identifier.citationKumar S, Chenthamarakshan V, Reddy MV, Narang P, Gupta OP, Harinath BC. Detection of tuberculous IgG antibodies using Mycobacterium tuberculosis H37Ra, excretory secretory antigen and tuberculin-purified protein derivative. Indian Journal of Experimental Biology. 1994 Mar; 32(3): 163-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/55670
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAntibodies, Bacterial --blooden_US
dc.subject.meshAntigens, Bacterial --immunologyen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --blooden_US
dc.subject.meshMycobacterium tuberculosis --immunologyen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSputum --microbiologyen_US
dc.subject.meshTuberculin --immunologyen_US
dc.subject.meshTuberculosis --blooden_US
dc.titleDetection of tuberculous IgG antibodies using Mycobacterium tuberculosis H37Ra, excretory secretory antigen and tuberculin-purified protein derivative.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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