An improved method for chemical devitellinization of X-gal stained Drosophila embryos.

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dc.contributor.authorSingh, Aen_US
dc.contributor.authorKango, Men_US
dc.contributor.authorSinha, Pen_US
dc.date.accessioned2009-05-28T14:39:39Z
dc.date.available2009-05-28T14:39:39Z
dc.date.issued1995-02-01en_US
dc.description.abstractIn Drosophila developmental biological studies, X-gal staining is commonly employed to study the spatio-temporal expression of the lacZ reporter gene in the transformed flies or their embryos. Study of the lacZ pattern in embryos often suffers from the lack of an efficient and high yielding technique for devitellinization of X-gal stained embryos. Devitellinization techniques employed during antibody staining, in situ hybridization or embryonic cuticular preparations generally do not give satisfactory results when used for similar purpose in X-gal stained embryos. This results in the flaky appearance of the blue stain. We present here an improved chemical devitellinization technique which gives a high yield of devitellinized embryos and a better resolution of the X-gal staining pattern.en_US
dc.description.affiliationDrosophila Stock Center, School of Life Science, Indore, India.en_US
dc.identifier.citationSingh A, Kango M, Sinha P. An improved method for chemical devitellinization of X-gal stained Drosophila embryos. Indian Journal of Experimental Biology. 1995 Feb; 33(2): 150-2en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60996
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDrosophila --drug effectsen_US
dc.subject.meshEmbryo, Nonmammalian --drug effectsen_US
dc.subject.meshGalactosidesen_US
dc.subject.meshIndolesen_US
dc.subject.meshStaining and Labelingen_US
dc.subject.meshVitelline Membrane --drug effectsen_US
dc.titleAn improved method for chemical devitellinization of X-gal stained Drosophila embryos.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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