Monoxenic in vitro production and colonization potential of AM fungus Glomus intraradices.

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dc.contributor.authorMohamma, Aen_US
dc.contributor.authorKhan, A Gen_US
dc.date.accessioned2009-05-28T15:58:04Z
dc.date.available2009-05-28T15:58:04Z
dc.date.issued2002-09-18en_US
dc.description.abstractThe paper reports the establishment of mycorrhizal infection of a non-mycorrhizal Ri-T-DNA transformed carrot root when co-cultured with a surface sterilized sweet potato root segment colonized by arbuscular mycorrhizal (AM) fungus G. intraradices on minimal M medium. Extensive fungal hyphal emergence from each cut end of the mycorrhizal sweet potato root piece was observed in one week old cultures. These hyphae caused infection on contacting the transformed-carrot- root segment and produced many hyphae and spores both inside and outside the zone of the root after 6 week of growth. Axenically produced fungal propagules proliferated on the surface of fresh minimal M medium when sub-cultured without any root segment. On repeated sub-culturing, these propagules did not lose their ability to grow and produced many juvenile small spore-like vesicles during the non-symbiotic phase. Although these spores were morphologically and anatomically similar to their soil borne counter parts, they were much smaller. When placed in the vicinity of a fresh hairy root on the minimal medium or a Sudan grass seedling in sand culture, the axenically produced AM fungal propagules caused root infection, but the infection characteristics were significantly different to the original culture in terms of shape (spherical vs oval) and size (20 microm vs 45 microm) of the intraradical vesicles, and absence of 'H' branches. Sudan grass seedlings inoculated with the axenically cultured fungus showed significantly (P < 0.05) higher dry weights plant'. When compared to the plants inoculated with sand cultures, the growth parameters and the percentage infection were not significantly different. However, when both sources of inocula were used together, a synergistic effect on plant growth as well as root infection was observed.en_US
dc.description.affiliationSchool of Science, Food & Horticulture, College of Science, Technology & Environment, University of Western Sydney, Locked Bag 1797, Penrith South DC NSW 1797, Australia.en_US
dc.identifier.citationMohamma A, Khan AG. Monoxenic in vitro production and colonization potential of AM fungus Glomus intraradices. Indian Journal of Experimental Biology. 2002 Sep; 40(9): 1087-91en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/62641
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDNA, Bacterial --geneticsen_US
dc.subject.meshDaucus carota --microbiologyen_US
dc.subject.meshFungi --growth & developmenten_US
dc.subject.meshHyphaeen_US
dc.subject.meshMycorrhizae --growth & developmenten_US
dc.subject.meshPlant Diseasesen_US
dc.subject.meshPlant Roots --microbiologyen_US
dc.subject.meshPoaceae --chemistryen_US
dc.subject.meshRhizobiumen_US
dc.subject.meshSeedling --growth & developmenten_US
dc.subject.meshSolanum tuberosum --growth & developmenten_US
dc.subject.meshSpores, Fungal --growth & developmenten_US
dc.subject.meshSymbiosisen_US
dc.titleMonoxenic in vitro production and colonization potential of AM fungus Glomus intraradices.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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