Mast cells generate cysteinyl leukotrienes and interferon- as well as evince impaired IgE-dependent degranulation upon TLR7 engagement.

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dc.contributor.authorWitczak, P
dc.contributor.authorPietrzak, A
dc.contributor.authorWódz, K
dc.contributor.authorBrzezińska Błaszczyk, E
dc.date.accessioned2014-12-11T05:42:00Z
dc.date.available2014-12-11T05:42:00Z
dc.date.issued2014-06
dc.description.abstractMast cells are numerous at anatomical sites close to external environment, virtually at the portals of infection. A few data indicated that these cells express cytoplasmic Toll-like receptors (TLRs) recognizing virus-derived molecules. Accordingly, mast cells could participate in anti-viral defense or/and in viral-related diseases. However, data concerning the influence of viruses on mast cell activity are limited. Thus, the aim of our study was to determine mast cell response to TLR7 ligand, i.e. resiquimod (R848), a synthetic mimic of viral ssRNA. Since mast cells play a central role in allergic reactions the effect of TLR7 agonist was also investigated on FcεRI-dependent mast cell response. Experiments were carried out in vitro on freshly isolated fully mature rat peritoneal mast cells. Mast cells exhibit constitutive TLR7 molecule expression and its up-regulation after the agonist challenge. TLR7-mediated mast cell stimulation resulted in cysteinyl leukotriene (cysLT) and interferon (IFN)-β synthesis, whereas no histamine and CXCL8 secretion was stated. Moreover, mast cell priming with TLR7 ligand caused the reduction in anti-IgE-induced histamine release. The results suggest that ssRNA viruses could directly activate mast cells to alter their phenotype and to release of potent proinflammatory mediators or indirectly modulate IgE-dependent allergic processes.en_US
dc.identifier.citationWitczak P, Pietrzak A, Wódz K, Brzezińska Błaszczyk E. Mast cells generate cysteinyl leukotrienes and interferon- as well as evince impaired IgE-dependent degranulation upon TLR7 engagement. Indian Journal of Experimental Biology. 2014 Jun; 52(6): 589-596.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153737
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/28865en_US
dc.subjectInterferonsen_US
dc.subjectLeukotrienesen_US
dc.subjectMast cellsen_US
dc.subjectToll-like receptor 7en_US
dc.subjectViral infectionen_US
dc.subject.meshAnimals
dc.subject.meshCell Degranulation --drug effects
dc.subject.meshCells, Cultured
dc.subject.meshFemale
dc.subject.meshImidazoles --pharmacology
dc.subject.meshImmunoglobulin E --physiology
dc.subject.meshInterferon-beta --metabolism
dc.subject.meshLeukotrienes --metabolism
dc.subject.meshMast Cells --drug effects
dc.subject.meshMast Cells --immunology
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.subject.meshToll-Like Receptor 7 --agonists
dc.subject.meshToll-Like Receptor 7 --metabolism
dc.titleMast cells generate cysteinyl leukotrienes and interferon- as well as evince impaired IgE-dependent degranulation upon TLR7 engagement.en_US
dc.typeArticleen_US
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