Release of superoxide anion from activated mouse peritoneal macrophages during Mycobacterium tuberculosis infection.

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dc.contributor.authorKhanna, Men_US
dc.contributor.authorSrivastava, L Men_US
dc.date.accessioned2009-05-28T11:14:07Z
dc.date.available2009-05-28T11:14:07Z
dc.date.issued1996-05-01en_US
dc.description.abstractMouse peritoneal macrophage monolayers infected with M. tuberculosis were cultured in RPMI up to 7 days. Release of superoxide was assayed on different days in presence or absence of Phorbol myristate acetate (PMA), a known stimulator of NADPH oxidase which is involved superoxide production. Basal level of superoxide release was significantly higher in M. tuberculosis infected peritoneal mouse macrophages (P < 0.01) as compared to normal mouse macrophages. When normal and tuberculoid macrophage cultures were stimulated with PMA, increased superoxide anion release was observed in both the cultures but the increase of superoxide was significantly higher in normal macrophages as compared to tuberculoid stimulated macrophages. Superoxide release was maximum in 4 day old cultured macrophages and gradually it declined in older cultures by day 7, both in vitro and in vivo. A defective macrophage function in killing of M. tuberculosis bacilli was observed after 4 days of in vitro and in vivo cultures.en_US
dc.description.affiliationDepartment of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.en_US
dc.identifier.citationKhanna M, Srivastava LM. Release of superoxide anion from activated mouse peritoneal macrophages during Mycobacterium tuberculosis infection. Indian Journal of Experimental Biology. 1996 May; 34(5): 468-71en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56430
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshMacrophage Activation --drug effectsen_US
dc.subject.meshMacrophages, Peritoneal --drug effectsen_US
dc.subject.meshMiceen_US
dc.subject.meshSuperoxides --metabolismen_US
dc.subject.meshTetradecanoylphorbol Acetate --pharmacologyen_US
dc.subject.meshTuberculosis --metabolismen_US
dc.titleRelease of superoxide anion from activated mouse peritoneal macrophages during Mycobacterium tuberculosis infection.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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