Cryoethanolic Extraction, Purification of Allicin from Allium sativum and HPTLC analysis

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dc.contributor.authorManikyam, Hemanth Kumaren_US
dc.contributor.authorJ, Dhanaseelanen_US
dc.contributor.authorMani, Jayarajen_US
dc.date.accessioned2024-09-24T06:22:58Z
dc.date.available2024-09-24T06:22:58Z
dc.date.issued2024-06
dc.description.abstractThe significance of this research extends beyond the mere extraction and purification of allicin, encompassing its potential applications across diverse industries. Allicin exhibits a plethora of biological activities, including antimicrobial, antifungal, antiviral, and antiprotozoal properties. Its mechanism of action involves the inhibition of thiol-containing enzymes in microorganisms, rendering it effective against a wide array of pathogens. Moreover, allicin has demonstrated promising anticancer properties, eliciting apoptosis and inhibiting cell proliferation in various cancer cell lines. Additionally, its anti-inflammatory and cardioprotective effects underscore its potential therapeutic utility in mitigating cardiovascular diseases and other inflammatory conditions. This study presents a novel method for extracting and purifying allicin from raw garlic cloves. Dry ice ensures the ethanol to reach its cryo temperature (hilled to sub-zero temperatures ranging from -40癈 to -80癈). The process involves the use of ethanol, dry ice and ascorbic acid as solvents, vacuum stirring, and subsequent crystallization. The final product, a hygroscopic powder, was analyzed using High-Performance Thin-Layer Chromatography (HPTLC) to determine allicin content. The extraction yielded 5 grams of whitish hygroscopic powder from 21 grams of crude extract, with an allicin content of 5%. Chromatographic conditions included a mobile phase of acetonitrile: water: Formic acid (30:8:2) and derivatization with ninhydrin, with detection under UV light at 366 nm. This method provides an efficient way to isolate and purify allicin for various applications.en_US
dc.identifier.affiliationsDepartment of Chemistry, Faculty of Science, North East Frontier Technical University- Aalo Post Office, West Siang Distt, National Highway 229, Aalo, Arunachal Pradesh, 791001, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Adhiyamaan College of Engineering, Hosur, Tamil Nadu, 635109, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Plant Genetic Engineering and Molecular Biology Lab, Periyar University, Salem, Tamil Nadu-636011, Indiaen_US
dc.identifier.citationManikyam Hemanth Kumar, J Dhanaseelan, Mani Jayaraj. Cryoethanolic Extraction, Purification of Allicin from Allium sativum and HPTLC analysis . International Journal of Medical Research & Health Sciences. 2024-Jun; 33(6): 43-51en_US
dc.identifier.issn2231-086X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/226850
dc.languageenen_US
dc.publisherMs. M. B. Mondalen_US
dc.relation.issuenumber6en_US
dc.relation.volume33en_US
dc.source.urihttps://doi.org/10.9734/ijbcrr/2024/v33i6887en_US
dc.subjectAllicinen_US
dc.subjectallium sativumen_US
dc.subjectHPTLCen_US
dc.subjectextractionen_US
dc.subjectpurificationen_US
dc.subjectcrystallizationen_US
dc.subjectcryoethanolic extractionen_US
dc.titleCryoethanolic Extraction, Purification of Allicin from Allium sativum and HPTLC analysisen_US
dc.typeJournal Articleen_US
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