Activators and Inhibitors of α-glucosidase from Penicillium chrysogenum

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dc.contributor.authorEl-Shora, Hamed M.en_US
dc.contributor.authorIbrahim, Mohsen E.en_US
dc.contributor.authorAlfakharany, Mohammad W.en_US
dc.date.accessioned2019-12-06T09:17:31Z
dc.date.available2019-12-06T09:17:31Z
dc.date.issued2018-02
dc.description.abstractα-glucosidase (EC: 3.2.1.20) from Penicillium chrysogenum Thom ATCC 10106 was induced by GSH at the lower concentrations. H2O2 was inhibitor at all tested concentrations and the IC50 was 92.2%v/v. AMP, ADP and ATP enhanced the activity revealing that α-glucosidase is endothermic enzyme. The chelating agents are ethylenediaminetetraacetate (EDTA), α-α-dipyridyl and o-phenanthroline inhibited the enzyme. IC50 for these three compounds were 7.1, 10.2 and 10.9 mM, respectively. The highest activity of α-glucosidase was recorded at 150 mM phosphate buffer. Mannitol as polyol protected the enzyme against heat inactivation. The five sugars trehalose, lactose, raffinose, glucose and sucrose protected α-glucosidase against thermo-inactivation at 60ºC. Also, sarcosine as a product of glycine provided α-glucosidase with appreciable thermostability at 60ºC.en_US
dc.identifier.affiliationsDepartment of Botany, Faculty of Science, Mansoura University, Dakahlia, Egypt.en_US
dc.identifier.affiliationsDepartment of Botany, Faculty of Science, Port Said University, Port Said, Egypt.en_US
dc.identifier.affiliationsDepartment of Botany, Faculty of Science, Port Said University, Port Said, Egypt.en_US
dc.identifier.citationEl-Shora Hamed M., Ibrahim Mohsen E., Alfakharany Mohammad W.. Activators and Inhibitors of α-glucosidase from Penicillium chrysogenum. Annual Research & Review in Biology. 2018 Feb; 24(1): 1-9en_US
dc.identifier.issn2347-565X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/187797
dc.languageenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.issuenumber1en_US
dc.relation.volume24en_US
dc.source.urihttps://doi.org/10.9734/ARRB/2018/38408en_US
dc.subjectP chrysogenumen_US
dc.subjectglutathioneen_US
dc.subjectadenosine compoundsen_US
dc.subjectchelating agentsen_US
dc.subjecttrehaloseen_US
dc.subjectmannitolen_US
dc.titleActivators and Inhibitors of α-glucosidase from Penicillium chrysogenumen_US
dc.typeJournal Articleen_US
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