Modification by chloramphenicol of diethyl sulphate-induced male recombination frequency in Drosophila melanogaster.

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dc.contributor.authorMiglani, G Sen_US
dc.contributor.authorKaur, N Pen_US
dc.date.accessioned2009-05-28T15:57:03Z
dc.date.available2009-05-28T15:57:03Z
dc.date.issued1995-06-01en_US
dc.description.abstractTo study the effect of chloramphenicol (CPL, an inhibitor of protein synthesis) on diethyl sulphate (DES, a potent mutagen) induced male recombination frequency, the F1 (+/aristaless dumpy black cinnabar, al dp b cn) larvae of D. melanogaster were given a pre- or post-treatment of CPL with DES during the first or second half of larval life. In order to determine sensitivity of different germ cell stages to the induction and modification of male recombination frequency, five 3-day broods were taken from every F1 male. DES showed toxic effect on egg-to-adult development. DES was found to be a potent recombinogen. Several cases of non-reciprocal male recombination were recorded. The most frequent recombinant phenotype observed was b cn followed by cn and al. Majority of the recombinants appeared in clusters suggesting their pre-meiotic origin. DES produced male recombination at a stage where only primary spermatocytes were present in the larval testes. CPL when given as a pre- or post-treatment with DES revealed highest frequency of male recombination in broods that represented effect of treatment on spermatogonia predominantly. CPL enhanced the overall level of male recombination produced by DES in both pre- and post-treatments. The results suggested the role of protein synthesis in induction of male recombination in D. melanogaster. In addition, the present experiments give a methodology of enhancing the frequency of chemically-induced male recombination.en_US
dc.description.affiliationDepartment of Genetics, Punjab Agricultural University, Ludhiana, India.en_US
dc.identifier.citationMiglani GS, Kaur NP. Modification by chloramphenicol of diethyl sulphate-induced male recombination frequency in Drosophila melanogaster. Indian Journal of Experimental Biology. 1995 Jun; 33(6): 401-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/62620
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChloramphenicol --pharmacologyen_US
dc.subject.meshCrosses, Geneticen_US
dc.subject.meshDrosophila melanogaster --geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHomozygoteen_US
dc.subject.meshMaleen_US
dc.subject.meshMutagens --pharmacologyen_US
dc.subject.meshRecombination, Genetic --drug effectsen_US
dc.subject.meshSex Characteristicsen_US
dc.subject.meshSulfuric Acid Esters --pharmacologyen_US
dc.titleModification by chloramphenicol of diethyl sulphate-induced male recombination frequency in Drosophila melanogaster.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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