Ethanol extract of rosemary repressed pten expression in K562 culture cells.
Loading...
Date
2011-01
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Several medicinal herbs are found to have a potent transcriptional activity for the gene
involved in cellular function. In the present study, we aimed to determine the effects of such herbs on
PTEN (phosphatase and tensin homolog deleted on chromosome 10) gene, which is a candidate gene
involved in cancer development. The in vitro effect of a Rosemary (Rosmarinus officinalis) extract
on the reduction of PTEN expression was confirmed by reverse transcriptase-polymerase chain
reaction (RT-PCR). In addition, western blotting also confirmed the down-regulation of the protein at
dose dependent manner of the Rosemary extract. Our results show that component(s) of Rosemary
inhibits the expression of PTEN in K562 myeloid cell line cells.
Description
Keywords
Herb, Spice, Rosemary, Gene expression, PTEN, Cancer
Citation
Yoshida Hitomi, Okumura Naoko, Kitagishi Yasuko, Nishimura Yuri, Matsuda Satoru. Ethanol extract of rosemary repressed pten expression in K562 culture cells. International Journal of Applied Biology and Pharmaceutical Technology. 2011 Jan-Mar; 2(1): 316-322.