In vitro regeneration of Acacia catechu Willd. from callus and mature nodal explants--an improved method.

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dc.contributor.authorThakur, Manishaen_US
dc.contributor.authorSharma, D Ren_US
dc.contributor.authorKanwar, Kamleshen_US
dc.contributor.authorKant, Anilen_US
dc.date.accessioned2009-05-28T16:27:05Z
dc.date.available2009-05-28T16:27:05Z
dc.date.issued2002-07-25en_US
dc.description.abstractCallus was derived from cultured cotyledons on MS medium supplemented with 2,4-D (0.25 mg/l) and NAA (0.25 mg/l). Plantlets were regenerated from the callus and nodal explants on MS medium containing BAP (2.0 mg/l) and Kn (2.0 mg/l), and further multiplied on the same medium. Addition of adenine sulphate (25.0 mg/l), ascorbic acid (20.0 mg/l) and glutamine (150.0 mg/l) in the medium resulted in enhanced axillary branching. Multiple shoots formed after 6 weeks were separated and subcultured in the fresh medium of same composition. For rhizogenesis, microshoots of 2.0-2.5 cm length were dipped in sterilized IAA solution (10 mg/l) for 24 hr followed by transfer to half strength MS medium containing activated charcoal (0.02%) resulting in rooting (75%) within 8 weeks. The rooted plants were transferred to pots containing sterilized soil and sand mixture for hardening and 71% survival was recorded. Fifty true to type plantlets of A. catechu could be obtained within seven months of culture establishment.en_US
dc.description.affiliationDepartment of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, Nauni Solan 173 230, India.en_US
dc.identifier.citationThakur M, Sharma DR, Kanwar K, Kant A. In vitro regeneration of Acacia catechu Willd. from callus and mature nodal explants--an improved method. Indian Journal of Experimental Biology. 2002 Jul; 40(7): 850-3en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/63249
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAcacia --geneticsen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshRegenerationen_US
dc.titleIn vitro regeneration of Acacia catechu Willd. from callus and mature nodal explants--an improved method.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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