Pharmacokinetic study of niosome encapsulated insulin.

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dc.contributor.authorKhaksa, Gen_US
dc.contributor.authorD'Souza, Ren_US
dc.contributor.authorLewis, Sen_US
dc.contributor.authorUdupa, Nen_US
dc.date.accessioned2009-05-28T14:27:30Z
dc.date.available2009-05-28T14:27:30Z
dc.date.issued2000-09-04en_US
dc.description.abstractPharmacokinetic profile and hypoglycemic effect, after intraperitoneal injection of insulin and insulin encapsulated in niosomes were determined in diabetic rats. Niosomes (non-ionic surfactant vesicles) of different doses and different lipid compositions were prepared by lipid layer hydration method. Plasma samples were collected at specified time intervals and plasma concentration of insulin was determined by HPLC. Blood glucose level was estimated spectrophotometrically using commercial glucose assay kit. In vitro release and pharmacokinetic profile of niosomal formulation and free insulin were evaluated. Though there was a slight delay in the in vitro drug release due to cholesterol content in the niosomes, there was no difference between the two preparations when plasma levels were compared in vivo. Niosomes significantly reduced the blood glucose level in diabetic rats. Fall in blood glucose level was almost 92% of initial value. In case of the niosomal form the half-life of insulin was prolonged by 4 -5 hr in contrast to 2 hr for free drug. Niosomes maintained the plasma insulin level up to 12 hr, but free drug was cleared quickly. The area under the plasma concentration-time curve for niosomal forms was, 26.07 degrees +/- 0.99 mIU. hr/ml and for free insulin was 11.722 +/- 1.00 mIU. hr/ml. More than 80% of the drug was successfully encapsulated to give a formulation with sustained release characteristics. Entrapment efficiency increased with increasing lipid concentration and decreased with increasing drug concentration. The results showed that insulin entrapped in niosomes prolongs the existence of drug in the body therefore increasing its therapeutic value.en_US
dc.description.affiliationDepartment of Pharmaceutics, College of Pharmaceutical Sciences, Manipal 576 119, India.en_US
dc.identifier.citationKhaksa G, D'Souza R, Lewis S, Udupa N. Pharmacokinetic study of niosome encapsulated insulin. Indian Journal of Experimental Biology. 2000 Sep; 38(9): 901-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60734
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiopolymersen_US
dc.subject.meshBlood Glucose --analysisen_US
dc.subject.meshCattleen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshDiabetes Mellitus, Experimental --metabolismen_US
dc.subject.meshDrug Carriersen_US
dc.subject.meshHypoglycemic Agents --pharmacokineticsen_US
dc.subject.meshInjections, Intraperitonealen_US
dc.subject.meshInsulin --pharmacokineticsen_US
dc.subject.meshLiposomesen_US
dc.subject.meshMaleen_US
dc.subject.meshParticle Sizeen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshTissue Distributionen_US
dc.titlePharmacokinetic study of niosome encapsulated insulin.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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