Optimization of Media Constituents for the Production of Alkaline Protease from Bacillus Licheniformis.
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Date
2015-07
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Abstract
Production of alkaline protease by Bacillus licheniformis has been investigated under submerged
fermentation. The physical and chemical parameters influencing submerged fermentation were optimized. The
effect of incubation time, temperature, pH, carbon sources and nitrogen sources and additional nutrients on the
production of alkaline protease was characterized. The optimum conditions for the protease production by Bacillus
licheniformis were found to be at pH 9.0 and temperature at 40ÂșC. The outcome of carbon and inorganic nitrogen
sources on protease production proved that glucose and casein were the effective medium ingredients for Bacillus
licheniformis respectively. The maximum amount of protease production was recorded in medium supplemented
with ammonium sulphate. Among the tested metal ions, the level of protease yield was found to be high in medium
supplemented with magnesium chloride. The protease production was amplified in the presence of 1.5% sodium
chloride. The extreme stability towards Triton X-100, Tween 20 and SDS was observed in Bacillus licheniformis
alkaline protease.
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Keywords
Protease, Bacillus licheniformis and surfactant
Citation
Nawas P Mohideen Askar, Banu V N Hameetha, Mohamed S Peer, Sundaram V Meenakshi, Sekaran M Chandhira. Optimization of Media Constituents for the Production of Alkaline Protease from Bacillus Licheniformis. International Journal of Applied Biology and Pharmaceutical Technology. 2015 Jul-Sept; 6(3): 169-175.