Effectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production.

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dc.contributor.authorSaha-Roy, Santaen_US
dc.contributor.authorShende, Nirajen_US
dc.contributor.authorKumar, Satishen_US
dc.contributor.authorHarinath, B Cen_US
dc.date.accessioned2009-05-28T11:37:02Z
dc.date.available2009-05-28T11:37:02Z
dc.date.issued2005-12-20en_US
dc.description.abstractMonospecific antibodies have been successfully utilized in antigen detection, which is better indicator of active infection. Mycobacterium tuberculosis excretory secretory (M tb ES) antigens such as ES 31, ES 41 and ES 43 (31 kDa, 41 kDa and 43 kDa protein, respectively) have been shown to be present in Mycobacterium tuberculosis H37Ra culture filtrate and are of diagnostic interest. To study the immunogenic potential of crude versus purified antigen, goat was immunized with M tb detergent soluble sonicate (DSS) antigen as well as purified antigen fraction (ESAS 7) containing ES 31 antigen. Both anti-DSS IgG antibody and anti ESAS 7 IgG antibody were found to be reactive with ES 31 antigen upto 1 ng concentration of antibody by ELISA. Crude DSS antigen was found to be quite effective in producing high titre antibodies and showed further high reactivity with other ES antigens (ES 41 and ES 43) of diagnostic interest.en_US
dc.description.affiliationJamnalal Bajaj Tropical Disease Research Centre and Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha 442 102, India.en_US
dc.identifier.citationSaha-Roy S, Shende N, Kumar S, Harinath BC. Effectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production. Indian Journal of Experimental Biology. 2005 Dec; 43(12): 1196-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56963
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial --biosynthesisen_US
dc.subject.meshAntigens, Bacterial --immunologyen_US
dc.subject.meshBacterial Proteins --immunologyen_US
dc.subject.meshGoats --immunologyen_US
dc.subject.meshMycobacterium --immunologyen_US
dc.subject.meshMycobacterium tuberculosis --immunologyen_US
dc.subject.meshTuberculosis Vaccines --immunologyen_US
dc.titleEffectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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