Effect of anthracycline antibiotics on in vitro transcription of chromatin in a Sarcoma-180 whole cell extract.

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dc.contributor.authorPanda, C Ken_US
dc.contributor.authorChakraborty, B Ken_US
dc.contributor.authorChoudhury, Ken_US
dc.date.accessioned1989-10-01en_US
dc.date.accessioned2009-05-27T09:09:36Z
dc.date.available1989-10-01en_US
dc.date.available2009-05-27T09:09:36Z
dc.date.issued1989-10-01en_US
dc.description.abstractA soluble extract capable of transcribing Sarcoma-180 chromatin and DNA in a cell-free transcription system was prepared from Sarcoma-180 mouse ascites tumour cells. Incorporation of [3H]UTP into trichloroacetic acid-precipitable fraction is (i) reduced by 50% on removing DNase I hypersensitive sites of chromatin and (ii) inhibited by DNA binding antitumour anthracyclines, suggesting that this cell-free assay represents true transcription of active genes of Sarcoma-180 chromatin. Preparation of this soluble extract from mouse ascites tumour cells thus presents a very convenient way of studying cell-free transcription of active genes of chromatin and effect of antitumour agents on chromatin transcription.en_US
dc.identifier.citationPanda CK, Chakraborty BK, Choudhury K. Effect of anthracycline antibiotics on in vitro transcription of chromatin in a Sarcoma-180 whole cell extract. Indian Journal of Biochemistry & Biophysics. 1989 Oct; 26(5): 293-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26640
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibiotics, Antineoplastic --pharmacologyen_US
dc.subject.meshCell-Free Systemen_US
dc.subject.meshChromatin --metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshSarcoma 180 --geneticsen_US
dc.subject.meshTranscription, Genetic --drug effectsen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleEffect of anthracycline antibiotics on in vitro transcription of chromatin in a Sarcoma-180 whole cell extract.en_US
dc.typeJournal Articleen_US
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