Effect of anthracycline antibiotics on in vitro transcription of chromatin in a Sarcoma-180 whole cell extract.
No Thumbnail Available
Date
1989-10-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A soluble extract capable of transcribing Sarcoma-180 chromatin and DNA in a cell-free transcription system was prepared from Sarcoma-180 mouse ascites tumour cells. Incorporation of [3H]UTP into trichloroacetic acid-precipitable fraction is (i) reduced by 50% on removing DNase I hypersensitive sites of chromatin and (ii) inhibited by DNA binding antitumour anthracyclines, suggesting that this cell-free assay represents true transcription of active genes of Sarcoma-180 chromatin. Preparation of this soluble extract from mouse ascites tumour cells thus presents a very convenient way of studying cell-free transcription of active genes of chromatin and effect of antitumour agents on chromatin transcription.
Description
Keywords
Citation
Panda CK, Chakraborty BK, Choudhury K. Effect of anthracycline antibiotics on in vitro transcription of chromatin in a Sarcoma-180 whole cell extract. Indian Journal of Biochemistry & Biophysics. 1989 Oct; 26(5): 293-5