Effect of different exposure periods to the infrared 1.48 m diode laser on the inner cell mass and trophectoderm of blastocysts.

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dc.contributor.authorPhophong, Phakphum
dc.contributor.authorManoch, Darapa
dc.contributor.authorChoavaratana, Roungsin
dc.date.accessioned2012-03-21T10:14:23Z
dc.date.available2012-03-21T10:14:23Z
dc.date.issued2002-11
dc.description.abstractThis study was carried out to determine the effect of the duration of exposure to an infrared 1.48 mm diode laser, on the number of cells in the inner cell mass and the trophectoderm of blastocysts following laser-assisted embryo biopsy. A total of 102 mouse embryos were used in the study. The embryos were randomly divided into three groups; group A (n = 22), group B (n = 47) and group C (n = 33). The embryos in group A were biopsied using the laser with an exposure of 600 ms, whereas those in group B were biopsied using the same laser with an exposure of 5 ms. The embryos in group C were incubated in culture without any procedures, as a control group. The blastocyst formation rates of group B (46/47, 97.8%) and group C (33/33, 100%) were significantly higher than that of group A (12/22, 54.5%). The number of cells in the inner cell mass, trophectoderm and the total number of cells in the blastocyst in group A (16.1 ฑ 5.1, 35.5 ฑ 10.9, 51.6 ฑ 12.9) were similar to those in group B (14.0 ฑ 5.6, 36.0 ฑ 12.7, 50.0 ฑ 18.3). The number of cells in the inner cell mass, trophectoderm and the total number of cells in the blastocysts in group C (19.1 ฑ 6.5, 45.8 ฑ 14.0, 65.0 ฑ 18.7) was significantly higher than those of the study groups. In conclusion, the longer duration of exposure to the infrared 1.48 mm diode laser might adversely affect blastocyst formation. However, it might not affect the quality of the blastocysts with regard to the number of cells in the inner cell mass and the trophectoderm.en_US
dc.identifier.citationPhophong Phakphum, Manoch Darapa, Choavaratana Roungsin. Effect of different exposure periods to the infrared 1.48 m diode laser on the inner cell mass and trophectoderm of blastocysts. Siriraj Medical Journal, 2002 Nov; 54(11): 698-703.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/137301
dc.language.isoenen_US
dc.source.urihttps://www.sirirajmedj.com/content_download.php?content_id=995en_US
dc.subjectDiode laseren_US
dc.subjectInner cell massen_US
dc.subjectTrophectodermen_US
dc.subjectBlastocystsen_US
dc.titleEffect of different exposure periods to the infrared 1.48 m diode laser on the inner cell mass and trophectoderm of blastocysts.en_US
dc.typeArticleen_US
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