White Spot Syndrome Virus infection in Penaeus monodon is facilitated by housekeeping molecules.
Date
2013-12
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Abstract
White Spot Syndrome Virus (WSSV) is a major pathogen in shrimp aquaculture, and its rampant spread has resulted
in great economic loss. Identification of host cellular proteins interacting with WSSV will help in unravelling the
repertoire of host proteins involved in WSSV infection. In this study, we have employed one-dimensional and twodimension
virus overlay protein binding assay (VOPBA) followed by matrix-assisted laser desorption ionization-time
of flight mass spectrometry (MALDI-TOF MS) to identify the host proteins of Penaeus monodon that could interact
with WSSV. The VOPBA results suggest that WSSV interacted with housekeeping proteins such as heat shock
protein 70, ATP synthase subunit β, phosphopyruvate hydratase, allergen Pen m 2, glyceraldehyde-3-phosphate
dehydrogenase, sarcoplasmic calcium-binding protein, actin and 14-3-3-like protein. Our findings suggest that WSSV
exploits an array of housekeeping proteins for its transmission and propagation in P. monodon.
Description
Keywords
ATP synthase β subunit, HSP70, virus–host interaction, virus overlay protein binding assay
Citation
Biradar Vinayak, Narwade Santosh, Paingankar Mandar, Deobagkar Deepti. White Spot Syndrome Virus infection in Penaeus monodon is facilitated by housekeeping molecules. Journal of Biosciences. 2013 Dec; 38(5): 917-924.