Isolation of Lumpy Skin Disease Virus from Andhra Prad

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dc.contributor.authorRanjith Reddy, Ren_US
dc.contributor.authorRani Prameela, Den_US
dc.contributor.authorVijaya Lakshmi, Sen_US
dc.contributor.authorLahari, Len_US
dc.contributor.authorSailaja, N.en_US
dc.date.accessioned2025-05-12T09:41:22Z
dc.date.available2025-05-12T09:41:22Z
dc.date.issued2024-06
dc.description.abstractA total of 31 blood samples and 31 skin scabs were collected from both adult and young calves of suspected cases of Lumpy skin disease showing clinical signs. All the collected samples (31 blood samples and 31 skin scabs) were processed and subjected for virus isolation in embryonated chicken eggs and primary testicular cell cultures. The skin scabs (7 out of 14) positive at genus and (amplifying P32 gene) species level amplifying (GPCR, RPO30 and Ankyrin repeat) genes by PCR were attempted for virus isolation on embryonated chicken eggs of 10-11 days old. All the seven virus isolates (TPT-1, TPT-2, K-1, K-2, G-1, CH-1, CH-2) showed hemorrhages on chorio-allantoic membrane at second passage level on 5th day of post inoculation whereas only three isolates (TPT-2, K-1, CH-1) showed characteristic pock lesions on 5 th day of post inoculation at 5 th passage level. These were further confirmed by PCR amplifying P32 gene. Out of these three isolates (TPT-2, K-1, CH-1) only two isolates (TPT-2, K-1) were subjected for adaptation in primary lamb testicular cell cultures and CPE was observed with shrinkage, cell rounding and cell aggregation between 48-72hrs post infection at first blind passage level.en_US
dc.identifier.affiliationsState Level Diagnostic Laboratory, SVVU, Tirupati, INDIAen_US
dc.identifier.affiliationsState Level Diagnostic Laboratory, SVVU, Tirupati, INDIAen_US
dc.identifier.affiliationsDepartment of Veterinary Microbiology, College of Veterinary Science, Tirupati, INDIAen_US
dc.identifier.affiliationsDepartment of Veterinary Pathology, College of Veterinary Science, Tirupati, INDIAen_US
dc.identifier.citationRanjith Reddy R, Rani Prameela D, Vijaya Lakshmi S, Lahari L, Sailaja N. . Isolation of Lumpy Skin Disease Virus from Andhra Prad. Journal of Animal Research. 2024 Jun; 14(3): 189-197en_US
dc.identifier.issn2249-6629
dc.identifier.issn2277-940X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/247440
dc.languageenen_US
dc.publisherThe Association of Mastitisen_US
dc.relation.issuenumber3en_US
dc.relation.volume14en_US
dc.source.urihttps://www.doi.org/10.30954/2277-940X.03.2024.3en_US
dc.subjectLumpy skin disease virusen_US
dc.subjectEmbryonated chicken eggsen_US
dc.subjectprimary testicular cell cultureen_US
dc.titleIsolation of Lumpy Skin Disease Virus from Andhra Praden_US
dc.typeJournal Articleen_US
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