Investigation of Genetic Diversity among Medicago species Using RAPD Markers.
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Date
2015
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Abstract
Aims: Medicago is known as the Queen of forage with potential economic importance to our
society. The present study aimed at the use of RAPD-PCR DNA marker to identify the genetic
fingerprints affinities of six species of Alfalfa.
Place and Duration of Study: The study was conducted at the Department of Genetics, Garden
Campus, Hazara University, Mansehra Pakistan during February, 2011 to August, 2013.
Methodology: In this study, six species of Medicago namely TWAL (Tetraploid Wisconsin Alfalfa
Line), Medicago arborea, Medicago falcata, Medicago sativa, Medicago lupulina and Medicago
polymorpha were used to explore the diversity of alfalfa. Seven out of 120 decamers produced 34
polymorphic loci with 100% polymorphism to identify the different species of Medicago crop. The range of polymorphic loci was observed from 300 to 700 bp. Eleven species specific loci were
generated by seven decamers. Primer B-18 generated single specific locus 700 bp against
genomic DNA of M. lupulina and it is important to identify particular species of Alfalfa. The bivariate
data were recorded as the presence of locus 1 and absence 0 and then this data was transferred
into A and C respectively to make it suitable for DNAMAN software (version 5.2.2.0; Applied
Biostatistics Inc). Moreover, cluster analysis was performed using sequence alignment and
divergence function of the DNAMAN against the bivariate data collected from the products of
decamers. All members clustered in a unique pattern except M. falcata and M. lupulina those
shared 86% homology. Three distinct groups were observed during UPGMA (Unweighted pair
Group Method with Arithmetic Mean). During the phylogenetic study, TWAL was observed to have
genetic diversity from other five species of Alfalfa.
Conclusion: So, the present study is enabling us to discriminate different species of Alfalfa and it
could be useful to identify and authenticate different species of the same genus of medicinal
important plant from the Flora of Pakistan.
Description
Keywords
Medicago, RAPD, Genetic diversity
Citation
Murtaza Komal, Muhammad Khushi, Alam Mukhtar, Khan Ayaz, Wahab Zainul, Nadeem Muhammad Shahid, Akbar Nazia, Ahmad Waqar, Ahmad Habib.Investigation of Genetic Diversity among Medicago species Using RAPD Markers. British Biotechnology Journal. 2015 6(2): 79-86.