Optimisation of fermentation conditions for gluconic acid production by a mutant of Aspergillus niger.

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dc.contributor.authorSingh, O Ven_US
dc.contributor.authorSharma, Aen_US
dc.contributor.authorSingh, R Pen_US
dc.date.accessioned2009-05-28T15:26:02Z
dc.date.available2009-05-28T15:26:02Z
dc.date.issued2001-11-22en_US
dc.description.abstractAspergillus niger ORS-4, isolated from the sugarcane industry waste materials was found to produce notable level of gluconic acid. From this strain, a mutant Aspergillus niger ORS-4.410 having remarkable increase in gluconic acid production was isolated and compared for fermentation properties. Among the various substrates used, glucose resulted into maximum production of gluconic acid (78.04 g/L). 12% concentration led to maximum production. Effect of spore age and inoculum level on fermentation indicated an inoculum level of 2% of the 4-7 days old spores were best suited for gluconic acid production. Maximum gluconate production could be achieved after 10-12 days of the fermentation at 30 degrees C and at a pH of 5.5. Kinetic analysis of production indicated that growth of the mutant was favoured during initial stages of the fermentation (4-8 days) and production increased during the subsequent 8-12 days of the fermentation. CaCO3 and varying concentrations of different nutrients affected the production of gluconic acid. Analysis of variance for the factors evaluated the significant difference in the production levels.en_US
dc.description.affiliationDepartment of Biosciences and Biotechnology, University of Roorkee, India.en_US
dc.identifier.citationSingh OV, Sharma A, Singh RP. Optimisation of fermentation conditions for gluconic acid production by a mutant of Aspergillus niger. Indian Journal of Experimental Biology. 2001 Nov; 39(11): 1136-43en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/61973
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAspergillus niger --geneticsen_US
dc.subject.meshFermentationen_US
dc.subject.meshGluconates --metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshMutationen_US
dc.subject.meshSpores, Fungal --geneticsen_US
dc.titleOptimisation of fermentation conditions for gluconic acid production by a mutant of Aspergillus niger.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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