Characteristics of monoclonal antibodies against porcine zona pellucida-3 and their functional relevance.

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dc.contributor.authorGupta, S Ken_US
dc.contributor.authorBagavant, Hen_US
dc.contributor.authorKoothan, P Ten_US
dc.contributor.authorTalwar, G Pen_US
dc.contributor.authorYurewicz, E Cen_US
dc.contributor.authorSacco, A Gen_US
dc.date.accessioned2009-05-28T14:25:44Z
dc.date.available2009-05-28T14:25:44Z
dc.date.issued1992-11-01en_US
dc.description.abstractSeven monoclonal antibodies (MAs) against 55 kDa glycoprotein family of porcine zona pellucida (ZP3) reacting with either ZP3 alpha (MA-7, MA-27, MA-28) or ZP3 beta (MA-1, MA-2, MA-11, MA-30) have been described. MA-1, -2, -27, -28 and -30 do not recognize carbohydrate determinants as shown by their reactivity to the deglycosylated (DG) ZP3 alpha and ZP3 beta. Indirect immunoperoxidase studies showed that all MAs reacted with zona pellucida from porcine and monkey ovaries. Only MA-1 and -27 reacted with ZP from rabbit ovary as well, while none of the MAs recognised mouse ZP, MA-7, -11, -27, -28 and -30 inhibited in vitro, the zona lysis by trypsin as well as the binding of ZP3 to sperm membrane vesicle as investigated by ELISA.en_US
dc.description.affiliationNational Institute of Immunology, Shahid Jeet Singh Marg, New Delhi.en_US
dc.identifier.citationGupta SK, Bagavant H, Koothan PT, Talwar GP, Yurewicz EC, Sacco AG. Characteristics of monoclonal antibodies against porcine zona pellucida-3 and their functional relevance. Indian Journal of Experimental Biology. 1992 Nov; 30(11): 1000-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60696
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonalen_US
dc.subject.meshEgg Proteinsen_US
dc.subject.meshFemaleen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshMacaca mulattaen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Glycoproteins --analysisen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB C --immunologyen_US
dc.subject.meshOvary --cytologyen_US
dc.subject.meshRabbitsen_US
dc.subject.meshReceptors, Cell Surfaceen_US
dc.subject.meshSperm-Ovum Interactionsen_US
dc.subject.meshSpermatozoa --physiologyen_US
dc.subject.meshSpleen --immunologyen_US
dc.subject.meshSwineen_US
dc.subject.meshZona Pellucida --physiologyen_US
dc.titleCharacteristics of monoclonal antibodies against porcine zona pellucida-3 and their functional relevance.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
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