Keratinase mediated fabrication and partial characterization of gold nanoparticles and its antibacterial potential
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Date
2023-01
Journal Title
Journal ISSN
Volume Title
Publisher
CSIR-NIScPR
Abstract
Keratinase is mainly involved in recycling of keratin waste. Of late, researchers extended its application to nanotechnology. In the present study, we have made an attempt to fabricate and characterize gold nanoparticles using crude keratinase enzyme from Serratia ficaria and also study their biological application, particularly antibacterial activity. The formation of gold nanoparticles (AuNPs) was first verified by UV-Visible Spectroscopy. FTIR spectra confirmed the presence of responsible secondary metabolites for stabilization of nanoparticles. The morphological characteristics and particle size of synthesized nanoparticles were analyzed. The AuNPs showed significant antibacterial activity against Klebsiella pneumonia, Bacillus cereus and Staphylococcus aureus. The highest radical scavenging activity, 60.62% for AuNPs was observed at 500 µg/mL. Results of this study reveals significance of keratinase application in nano-based biological applications.
Description
Keywords
Antibacterial activity, Antioxidant activity, Bio-nano synthesis, Waste utilization
Citation
Sudha Balraj, Athinath KS, Swabna Vivek, Sumathi Sundaravadivelu. Keratinase mediated fabrication and partial characterization of gold nanoparticles and its antibacterial potential. Indian Journal of Experimental Biology. 2023 Jan; 61(1): 59-65