Interobserver reproducibility in determining p16 overexpression in cervical lesions : use of a combined scoring method.

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dc.contributor.authorVinyuvat, Songkhunen_US
dc.contributor.authorKaralak, Ananten_US
dc.contributor.authorSuthipintawong, Cheepsumonen_US
dc.contributor.authorTungsinmunkong, Kobkulen_US
dc.contributor.authorKleebkaow, Pilaiwanen_US
dc.contributor.authorTrivijitsilp, Praserten_US
dc.contributor.authorSiriaunkgul, Sumaleeen_US
dc.contributor.authorTriratanachat, Surangen_US
dc.contributor.authorKhunamornpong, Surapanen_US
dc.contributor.authorChuangsuwanich, Tuenjaien_US
dc.contributor.authorSettakorn, Jongkolneeen_US
dc.date.accessioned2009-05-27T17:50:36Z
dc.date.available2009-05-27T17:50:36Z
dc.date.issued2008-10-05en_US
dc.descriptionAsian Pacific Journal of Cancer Prevention.en_US
dc.description.abstractOBJECTIVES: To evaluate interobserver reproducibility of a combined scoring method for immunohistochemical interpretation of p16 overexpression in cervical lesions. MATERIALS AND METHODS: p16 immunostaining was performed in cervical samples from 183 patients, including 69 normal, 42 low grade squamous intraepithelial lesions(LSIL), 36 high grade SIL (HSIL), and 36 squamous cell carcinomas(SCCAs). Each case was evaluated by a combined scoring method based on the percentage of positive cells (score 0-3), the intensity of staining (score 0-3), and the distribution pattern (score 0-2). Immunoexpression for p16 was considered as positive when the combined score was 4-8 and negative with a score of 0-3. Ten pathologists with varied experience in interpretating p16 immunostains evaluated each slide independently. RESULTS: All normal cervical squamous epithelia (69/69) were uniformly negative for p16. All HSILs (36/36), all SCCAs (100/100), and all but one of the LSILs (40/41, 97.6%) showed positive expression. In 172 of 183 cases (93.9%), p16 interpretation was concordant with all pathologists. Eleven cases with discordant results included 10 LSILs and 1 normal mucosa sample. Percentage of agreement of each pathologist pair ranged from 96.7-100% (mean 98.1%) with mean kappa value of 0.96 (range 0.93-1.000). CONCLUSION: The proposed combined scoring method shows good reproducibility among the participating pathologists and good correlation with the histologic diagnosis. This method may be a useful guide in the interpretation of p16 expression in cervical epithelial lesions.en_US
dc.description.affiliationInstitute of Pathology, Department of Medical Services, Ministry of Public Health, Bangkok, Thailand. songkhun@gmail.comen_US
dc.identifier.citationVinyuvat S, Karalak A, Suthipintawong C, Tungsinmunkong K, Kleebkaow P, Trivijitsilp P, Siriaunkgul S, Triratanachat S, Khunamornpong S, Chuangsuwanich T, Settakorn J. Interobserver reproducibility in determining p16 overexpression in cervical lesions : use of a combined scoring method. Asian Pacific Journal of Cancer Prevention. 2008 Oct-Dec; 9(4): 653-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/38003
dc.language.isoengen_US
dc.source.urihttps://www.apocp.orgen_US
dc.titleInterobserver reproducibility in determining p16 overexpression in cervical lesions : use of a combined scoring method.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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