Identification of T-Cell Epitopes in Proteins of Novel Human Coronavirus, SARS-Cov-2 for Vaccine Development
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Date
2020-04
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Applied Biology and Pharmaceutical Technology
Abstract
Recently, a novel human coronavirus, SARS-CoV-2 led to a worldwide serious health concern, causing severe respiratory tract infections in humans. It is the third highly pathogenic and transmissible coronavirus after severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in humans. The source of origin, transmission to humans and mechanisms associated with the pathogenicity of SARS-CoV-2 are not clear yet, however, its resemblance with SARS-CoV and several other bat coronaviruses was recently confirmed through genome sequencing related studies. It has been an emergent need to develop a potent and adequate number of drugs and vaccines to control the spread of coronavirus. We have screened the specific proteins such as ORF1ab polyprotein, surface glycoprotein, membrane glycoprotein and nucleocapsid phosphoprotein of SARS-CoV-2 for identification of T-cell epitopes using immunoinformatics tools. In this study we used different bioinformatics tools for analysis of genome and proteome. We retrieved gene sequence from NCBI. The expected molecular weight and isoelectric point (pI) values were also verified using Generunner and ExPaSy. These epitopes have showed the highest binding affinity with major histocompatibility complex (MHC) class I and II molecules. These findings may be useful as an immunodiagnostic tool for the development of peptide based novel vaccines.
Description
Keywords
SARS-CoV-2, Epitopes, MHC, Immunodiagnostic, Vaccine
Citation
Sahoo Biswajit, Kant Krishna, Rai Neeraj K., Chaudhary Dharmendra Kumar. Identification of T-Cell Epitopes in Proteins of Novel Human Coronavirus, SARS-Cov-2 for Vaccine Development. International Journal of Applied Biology and Pharmaceutical Technology. 2020 Apr; 11(2): 37-45